Influence of probiotics on water quality in intensified Litopenaeus vannamei ponds under minimum-water exchange

• Published in: AMB Express 2022-02, Vol.12 (1), p.22-22, Article 22
• Main Authors: Hassan, Marwa A., Fathallah, Mustafa A., Elzoghby, Mohamed A., Salem, Mohamed G., Helmy, Mohamed S.
• Format: Article
• Language: English
• Subjects: Ammonia; Bacillus; Biomedical and Life Sciences; Biotechnology; Degradation; Dissolved oxygen; Drinking water; Germination; Life Sciences; Litopenaeus vannamei; Magnetic field; Magnetic fields; Microbial Genetics and Genomics; Microbiology; Organic matter; Original; Original Article; pH effects; Ponds; Probiotics; Spores; Unionized ammonia; Water exchange; Water probiotics; Water quality; Water sampling
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-022-01370-5

The effects of two probiotics on NH 3 degradation, as well as the magnetic field (21.56 m tesla) on the germination and proliferation of Bacillus spores, were studied in-vitro. Additionally, the effect of these probiotics on water quality maintenance in Litopenaeus vannamei holding ponds was investigated. For 180 min, NH 3 degradation was assessed as follows: Set 1: ammonia-free tap water with NH 3 ; Probiotic A (5 × 10 10 viable Bacillus spores/g) with NH 3 ; Probiotic B (multi spp. 2 × 10 9  CFU/g) with NH 3 ; and Set 2: same as set 1 with 30 mg L −1 OM. The magnetic field was tested on Probiotic A (3.5 × 10 7 CFU) for 36 h in triplicate. In the presence of organic matter, both probiotics degrade NH 3 . The viable Bacillus count increased within 6 h of being exposed to the magnetic field, reaching its peak after 36 h. Firstly, fifteen ponds (250,000 PL/acre) were investigated, then 360 water samples were collected from the same corresponding pond for 8 weeks, and subjected to T1: control; T2: Probiotic A (0.007 g/m 3 /2 weeks); T3: Probiotic B (0.03 g/m 3 /2 weeks). Both probiotics with TVC and NH 3 demonstrated a negative correlation, on the other hand, they showed a significant (P ≤ 0.01) improvement in DO and pH. Overall, both probiotics were able to degrade NH 3 and the magnetic field (21.56 m tesla) was efficient to improve the germination and proliferation of Bacillus spores in-vitro. Probiotics were also effective for reducing TVC and NH 3 levels by increasing dissolved oxygen and pH in pond water.