Purines enrich root-associated Pseudomonas and improve wild soybean growth under salt stress

• Published in: Nature communications 2024-04, Vol.15 (1), p.3520-14, Article 3520
• Main Authors: Zheng, Yanfen, Cao, Xuwen, Zhou, Yanan, Ma, Siqi, Wang, Youqiang, Li, Zhe, Zhao, Donglin, Yang, Yanzhe, Zhang, Han, Meng, Chen, Xie, Zhihong, Sui, Xiaona, Xu, Kangwen, Li, Yiqiang, Zhang, Cheng-Sheng
• Format: Article
• Language: English
• Subjects: 38/23; 38/70; 38/91; 631/158/2456; 631/326/171/1818; 631/326/2565/2134; Abiotic stress; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Chemotaxis; Chemotaxis - genetics; CheW protein; Enrichment; Environmental stress; Exudation; Flagella; Glycine; Glycine max - genetics; Glycine max - metabolism; Glycine max - microbiology; Glycine soja; Humanities and Social Sciences; Metabolites; Metagenomics; Microbiota; Microbiota - drug effects; Microorganisms; Motility; multidisciplinary; Plant bacterial diseases; Plant Roots - metabolism; Plant Roots - microbiology; Pseudomonas; Pseudomonas - genetics; Pseudomonas - metabolism; Purines; Purines - metabolism; Purines - pharmacology; Rhizosphere; Salinity tolerance; Salt Stress - genetics; Salt tolerance; Salt Tolerance - genetics; Science; Science (multidisciplinary); Soil Microbiology; Soybeans; Xanthine - metabolism; Xanthines
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-47773-9

The root-associated microbiota plays an important role in the response to environmental stress. However, the underlying mechanisms controlling the interaction between salt-stressed plants and microbiota are poorly understood. Here, by focusing on a salt-tolerant plant wild soybean ( Glycine soja ), we demonstrate that highly conserved microbes dominated by Pseudomonas are enriched in the root and rhizosphere microbiota of salt-stressed plant. Two corresponding Pseudomonas isolates are confirmed to enhance the salt tolerance of wild soybean. Shotgun metagenomic and metatranscriptomic sequencing reveal that motility-associated genes, mainly chemotaxis and flagellar assembly, are significantly enriched and expressed in salt-treated samples. We further find that roots of salt stressed plants secreted purines, especially xanthine, which induce motility of the Pseudomonas isolates. Moreover, exogenous application for xanthine to non-stressed plants results in Pseudomonas enrichment, reproducing the microbiota shift in salt-stressed root. Finally, Pseudomonas mutant analysis shows that the motility related gene cheW is required for chemotaxis toward xanthine and for enhancing plant salt tolerance. Our study proposes that wild soybean recruits beneficial Pseudomonas species by exudating key metabolites (i.e., purine) against salt stress. Root-associated microbiota confers benefits to plant in responding to environmental stress. Here, the authors show that wild soybean secretes purines under salt stress, reshapes the microbiota and recruits Pseudomonas.