Gene expression profiling in patients with polymyalgia rheumatica before and after symptom-abolishing glucocorticoid treatment

The pathophysiology, including the impact of gene expression, of polymyalgia rheumatica (PMR) remains elusive. We profiled the gene expression in muscle tissue in PMR patients before and after glucocorticoid treatment. Gene expression was measured using Affymetrix Human Genome U133 Plus 2.0 arrays i...

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Bibliographic Details
Published in:BMC musculoskeletal disorders 2017-08, Vol.18 (1), p.341-341, Article 341
Main Authors: Kreiner, Frederik Flindt, Borup, Rehannah, Nielsen, Finn Cilius, Schjerling, Peter, Galbo, Henrik
Format: Article
Language:English
Subjects:
Age
Aged
Aged, 80 and over
Arthritis
Bioinformatics
Biopsy
C-reactive protein
Care and treatment
Corticosteroids
Cytokines
Disease
DNA microarray
DNA microarrays
Dosage and administration
Erythrocyte sedimentation rate
Extracellular matrix
Female
Follow-Up Studies
Gene expression
Gene Expression Profiling - methods
Gene regulation
Genomes
Genomics
Glucocorticoids
Glucocorticoids - therapeutic use
Humans
Hybridization
Interleukin 6
Interleukin-6 - metabolism
Male
Microarray Analysis
Middle Aged
Muscle
Musculoskeletal diseases
Musculoskeletal system
Pain
Patients
Physiological aspects
Polymerase chain reaction
Polymyalgia rheumatica
Polymyalgia Rheumatica - drug therapy
Polymyalgia Rheumatica - pathology
Prednisolone
Prednisolone - therapeutic use
Protein biosynthesis
Real-Time Polymerase Chain Reaction
Rheumatology
RNA polymerase
Superficial Back Muscles - pathology
Transcription
Tumor necrosis factor-TNF
Urine
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Summary:The pathophysiology, including the impact of gene expression, of polymyalgia rheumatica (PMR) remains elusive. We profiled the gene expression in muscle tissue in PMR patients before and after glucocorticoid treatment. Gene expression was measured using Affymetrix Human Genome U133 Plus 2.0 arrays in muscle biopsies from 8 glucocorticoid-naive patients with PMR and 10 controls before and after prednisolone-treatment for 14 days. For 14 genes, quantitative real-time PCR (qRT-PCR, n = 9 in both groups) was used to validate the microarray findings and to further investigate the expression of genes of particular interest. Prednisolone normalized erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in PMR patients. A total of 165 putatively clinically relevant, differentially expressed genes were identified (cut-off: fold difference > ±1.2, difference of mean > 30, and p 
ISSN:1471-2474
1471-2474
DOI:10.1186/s12891-017-1705-z