Gut microbiome diversity of porcine peritonitis model of sepsis

Animal models are essential in understanding of the mechanisms of sepsis moreover the development and the assessment of emerging therapies. In clinically relevant porcine model, however, a significant variability in the host response has been observed among animals. Thus, there is a strong demand to...

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Bibliographic Details
Published in:Scientific reports 2022-10, Vol.12 (1), p.17430-17430, Article 17430
Main Authors: Chalupova, Miroslava, Horak, Jan, Kramna, Lenka, Nalos, Lukas, Stengl, Milan, Chudejova, Katerina, Kraftova, Lucie, Cinek, Ondrej, Klein, Pavel, Matejovic, Martin, Hrabak, Jaroslav
Format: Article
Language:English
Subjects:
631/326/2565/2134
692/308/2778
692/308/409
692/308/575
Animal models
Animals
Bacteria - genetics
Digestive system
DNA, Ribosomal - genetics
Gastrointestinal Microbiome - genetics
Heterogeneity
Humanities and Social Sciences
Intestinal microflora
Microbiomes
Microbiota
multidisciplinary
Peritonitis
RNA, Ribosomal, 16S - genetics
rRNA 16S
Science
Science (multidisciplinary)
Seasons
Sepsis
Swine
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Summary:Animal models are essential in understanding of the mechanisms of sepsis moreover the development and the assessment of emerging therapies. In clinically relevant porcine model, however, a significant variability in the host response has been observed among animals. Thus, there is a strong demand to better understand the potential sources of this heterogeneity. In this study, we compared faecal microbiome composition of 12 animals. Three samples were collected at different time points from each animal. Bacteriome was subjected to 16S rDNA profiling. A significant difference in bacterial composition was associated with the season (p  0.3). The season batch explained 55% of the total variance in the bacteriome diversity. The season term was highly significant from the high-resolution level of the bacterial amplicon sequencing variants up to the level of phylum. The diversity of the microbiome composition could significantly influence experimental model of sepsis, and studies are warranted to demonstrate the effects of gut microbiome diversity on the host-response. If confirmed, control of the gut microbiome should become a standard part of the pre-clinical sepsis experiments.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-21079-6