LC-MS/MS and LC-UV Determination of Moniliformin by Adding Lanthanide Ions to the Mobile Phase

An innovative chromatographic analysis was developed for the determination of moniliformin (MON). Because of its ionic nature, MON is weakly retained in reversed-phase chromatography and the separation may be tricky. Nevertheless, this technique is normally used either with the formation of ion pair...

Full description

Saved in:
Bibliographic Details
Published in:Toxins 2019-09, Vol.11 (10), p.570
Main Authors: Bertuzzi, Terenzio, Rastelli, Silvia, Mulazzi, Annalisa, Pietri, Amedeo
Format: Article
Language:English
Subjects:
Anion exchange
Anion exchanging
Aqueous solutions
Chromatography
Coordination compounds
High performance liquid chromatography
Hydrophobicity
Ion pairs
Ions
Ketones
lanthanide complexes
lc-ms/ms
lc-uv
Ligands
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Moniliformin
Particle size
Retention
Separation
Ultraviolet radiation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An innovative chromatographic analysis was developed for the determination of moniliformin (MON). Because of its ionic nature, MON is weakly retained in reversed-phase chromatography and the separation may be tricky. Nevertheless, this technique is normally used either with the formation of ion pairs or employing specific RP columns for polar compounds, or combining anion exchange and hydrophobic interactions. Hydrophilic interaction chromatography (HILIC) was also used, but a non-negligible peak tailing was observed. Besides its ionic nature, MON is a di-ketone and di-ketones, mainly β-di-ketones, can easily form complexes with lanthanide ions. Then, in this work the addition of lanthanide ions to the mobile phase was investigated, aiming at improving peak shape and MON separation. La , Tb or Eu aqueous solutions were used as mobile phase and MON was chromatographed using a LC-NH column. The probable formation of coordination complexes lanthanide-MON in the HPLC mobile phase allowed to obtain a symmetrical peak shape and a satisfactory chromatographic separation by both mass spectrometry (MS/MS) and UV detection. Finally, a suitable extraction and purification method for MON determination in cereal samples was developed.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins11100570