Effect of CPP-ACP on remineralization of artificial caries-like lesion: an in situ study

• Published in: Brazilian oral research 2020-01, Vol.34, p.e061-e061
• Main Authors: OLIVEIRA, Patrícia Regina Almeida de, BARBOZA, Caroliny Mello, BARRETO, Luísa Schubach da Costa, TOSTES, Mônica Almeida
• Format: Article
• Language: English
• Subjects: Dental Enamel; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Fluorides; Hardness Tests; Tooth Remineralization
• ISSN: 1806-8324; 1807-3107; 1807-3107
• DOI: 10.1590/1807-3107bor-2020.vol34.0061

Abstract The purpose of this double-blind, randomized, crossover in situ study is to compare remineralization of preformed enamel lesions by casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) and fluoride dentifrice products. During each of four 10-day experimental legs, 10 participants wore intraoral removable palatal acrylic appliances with four human enamel slabs with preformed lesions. A 0.03-mL treatment paste was dripped extraorally onto the enamel blocks once a day for 3 min. The four randomly allocated treatments were as follows: CO- Control: silica dentifrice without fluoride; MP: MI Paste; MPP: MI Paste Plus and FD: Fluoride dentifrice - 1100 ppm F as NaF). Knoop surface hardness (SH) test was performed in three stages (T0 – sound enamel, T1 - after preformed lesion, and T2 - after treatment) and the cross-sectional hardness (CSH) test was performed after treatment using a 50-gram Knoop load for 15 s. Knoop hardness number (KHN) was similar between treatments. %SHr was significantly higher in the MP, FD, and MPP when compared to CO group (Kruskal-Wallis and Mann-Whitney tests, p < 0.05). Harder enamel was found in MP (75 μm) and FD groups at 75 to 175 μm. Treatment with DF, MP, and MPP promoted an increase of 20.27%, 19.24%, and 14.71%, respectively, in Integral Hardness Change (ΔIHC) when compared to CO (p