Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown...

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Published in:Npj genomic medicine 2022-06, Vol.7 (1), p.39-39, Article 39
Main Authors: Koeck, Rebekka M., Busato, Florence, Tost, Jorg, Consten, Dimitri, van Echten-Arends, Jannie, Mastenbroek, Sebastiaan, Wurth, Yvonne, Remy, Sylvie, Langie, Sabine, Nawrot, Tim S., Plusquin, Michelle, Alfano, Rossella, Bijnens, Esmée M., Gielen, Marij, van Golde, Ron, Dumoulin, John C. M., Brunner, Han, van Montfoort, Aafke P. A., Zamani Esteki, Masoud
Format: Article
Language:English
Subjects:
631/208/176/1988
692/308/409
Biochemistry, Molecular Biology
Bioinformatics
Biomedical and Life Sciences
Biomedicine
Birth weight
Cord blood
CpG islands
Culture media
DNA methylation
Embryos
Epigenetics
Fertilization
Gene Function
Gene Therapy
Genetic testing
Genetics
Genomes
Genomics
Gestational age
Human Genetics
In vitro fertilization
Internal Medicine
Life Sciences
Medicine
Neonates
Nutrition
Obstetrics
Premature birth
Reproductive health
Stochasticity
Toxicology
Umbilical cord
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Summary:A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.
ISSN:2056-7944
2056-7944
DOI:10.1038/s41525-022-00310-3