Cytogenomic evaluation of regenerated Aralia elata using PLOP-FISH and flow cytometry

Aralia elata is closely related to Panax ginseng and contains high levels of saponins and other medicinal compounds. Successful A. elata micropropagation is commercially significant; however, the genomic stability of tissue culture-derived regenerants is unclear. In this study, callus-derived regene...

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Bibliographic Details
Published in:Scientific reports 2024-12, Vol.14 (1), p.30289-9
Main Authors: Peniton, Eliazar Alumbro, Nguyen, Hong Thi, Waminal, Nomar Espinosa, Yang, Tae-Jin, Kim, Hyun Hee
Format: Article
Language:English
Subjects:
631/449/2491
631/449/2669
Aralia - genetics
Aralia elata
Callus
Chromosomes
Chromosomes, Plant - genetics
Cytogenetics
Cytogenomic study
Deoxyribonucleic acid
DNA
DNA probes
DNA, Plant - genetics
FISH
Flow cytometry
Flow Cytometry - methods
Fluorescence in situ hybridization
Fluorescent indicators
Genome Size
Genome, Plant
Genomes
Genomics
Genomics - methods
Humanities and Social Sciences
In Situ Hybridization, Fluorescence - methods
Medicinal plants
Micropropagation
multidisciplinary
Oligonucleotides
Ploidies
Ploidy
Probes
Regenerated plants
Regeneration - genetics
Saponins
Science
Science (multidisciplinary)
Tandem Repeat Sequences - genetics
Tissue culture
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Summary:Aralia elata is closely related to Panax ginseng and contains high levels of saponins and other medicinal compounds. Successful A. elata micropropagation is commercially significant; however, the genomic stability of tissue culture-derived regenerants is unclear. In this study, callus-derived regenerated A. elata plants were obtained, and their cytogenomic constitutions were assessed. Using RepeatExplorer, pre-labeled oligonucleotide probes (PLOPs) were developed with newly mined tandem repeats from
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-75004-0