Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflamm...

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Published in:Animals (Basel) 2020-06, Vol.10 (6), p.990
Main Authors: Slama, Petr, Kabourkova, Eliska, Sladek, Zbysek, Zavadilova, Terezie, Kratochvilova, Lucie, Kharkevich, Kristina, Roychoudhury, Shubhadeep, Pavlik, Ales, Roztocilova, Andrea, Uhrincat, Michal, Tancin, Vladimir, Kimura, Kazuhiro, Konecny, Roman, Kiku, Yoshio, Watanabe, Atsushi, Kwak, Jong- Young, Zouharova, Monika
Format: Article
Language:English
Subjects:
Apoptosis
Cattle
Escherichia coli
lipopolysaccharide
Lipopolysaccharides
lymphocyte
Lymphocytes
Mammary glands
mastitis
muramyl dipeptide
Observations
Peptides
Physiological aspects
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Summary:The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani10060990