The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb

Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication...

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Published in:eLife 2017-12, Vol.6
Main Authors: Reshef, Ronen, Kudryavitskaya, Elena, Shani-Narkiss, Haran, Isaacson, Batya, Rimmerman, Neta, Mizrahi, Adi, Yirmiya, Raz
Format: Article
Language:English
Subjects:
adult neruogenesis
Brain research
Cerebrospinal fluid
Chemical communication
Chemokines
CX3CR1 protein
Cytokines
Dendritic spines
development
Immunology
Macrophage colony-stimulating factor
Medical research
Microglia
Mitral cells
Neurogenesis
Neurons
Neuroscience
Neurosciences
Newborn infants
Odor
Olfactory bulb
Physiology
Proteins
Rodents
spines
Synapses
Synaptogenesis
Time
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Summary:Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient ( ) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between -deficient microglia and abGCs' dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.30809