Transcriptome-wide N6-methyladenosine modification profiling of long non-coding RNAs during replication of Marek's disease virus in vitro

The newly discovered reversible N6-methyladenosine (m A) modification plays an important regulatory role in gene expression. Long non-coding RNAs (lncRNAs) participate in Marek's disease virus (MDV) replication but how m A modifications in lncRNAs are affected during MDV infection is currently...

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Bibliographic Details
Published in:BMC genomics 2021-04, Vol.22 (1), p.296-296, Article 296
Main Authors: Sun, Aijun, Zhu, Xiaojing, Liu, Ying, Wang, Rui, Yang, Shuaikang, Teng, Man, Zheng, Luping, Luo, Jun, Zhang, Gaiping, Zhuang, Guoqing
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Animals
Chick Embryo
Chickens - genetics
Cluster analysis
DNA methylation
Embryos
Encyclopedias
Epigenetics
Gene expression
Gene sequencing
Genomes
Genomics
Herpesvirus 2, Gallid
Immunoprecipitation
Infections
KEGG
Long non-coding RNA
m6A
Marek Disease - genetics
Marek's disease
Marek’s disease virus
MeRIP-Seq
MicroRNAs
Mutation
N6-methyladenosine
Non-coding RNA
Pathogenesis
Proteins
Replication
RNA polymerase
RNA, Long Noncoding - genetics
Transcriptome
Transcriptomes
Viral infections
Virus Replication
Viruses
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Summary:The newly discovered reversible N6-methyladenosine (m A) modification plays an important regulatory role in gene expression. Long non-coding RNAs (lncRNAs) participate in Marek's disease virus (MDV) replication but how m A modifications in lncRNAs are affected during MDV infection is currently unknown. Herein, we profiled the transcriptome-wide m A modification in lncRNAs in MDV-infected chicken embryo fibroblast (CEF) cells. Methylated RNA immunoprecipitation sequencing results revealed that the lncRNA m A modification is highly conserved with MDV infection increasing the expression of lncRNA m A modified sites compared to uninfected cell controls. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that lncRNA m A modifications were highly associated with signaling pathways associated with MDV infection. In this study, the alterations seen in transcriptome-wide m A occurring in lncRNAs following MDV-infection suggest this process plays important regulatory roles during MDV replication. We report for the first time profiling of the alterations in transcriptome-wide m A modification in lncRNAs of MDV-infected CEF cells.
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-021-07619-w