Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice

The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers – HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant...

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Bibliographic Details
Published in:Czech Journal of Genetics and Plant Breeding 2017-01, Vol.53 (2), p.69-75
Main Authors: MARKIN, Nikolay, USATOV, Alexander, MAKARENKO, Maksim, AZARIN, Kiril, GORBACHENKO, Oleg, KOLOKOLOVA, Natalia, USATENKO, Tatyana, MARKINA, Olga, GAVRILOVA, Vera
Format: Article
Language:English
Subjects:
Breeding methods
cytoplasmic male sterility (cms)
Deoxyribonucleic acid
DNA
dna-based markers
Fertility
fertility restorer genes
Gene polymorphism
Genotypes
Helianthus
Markers
Pollen
Polymerase chain reaction
Polymorphism
Rf1 gene
Species
Sunflowers
testing system
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Summary:The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers – HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant character of the HRG01 marker and HRG01 amplicon polymorphism has been determined. Five annual and twenty-six perennial species of sunflower were tested for the presence of Rf genotypes. HRG02 proved to be a more appropriate marker for Rf1 determination in perennial species, and HRG01 was more informative for annual species. We have also developed the multiplex RT-PCR test system, which allows simultaneously detecting the dominant allele of Rf1 and CMS-PET1 associated mitotype.
ISSN:1212-1975
1805-9325
DOI:10.17221/108/2016-CJGPB