Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria

Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system's primary function is to hold these growth factors at the wound's site for a prolonged time and provide initial support for cells to attach and elaborate the extrac...

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Bibliographic Details
Published in:Journal of functional biomaterials 2020-12, Vol.11 (4), p.87
Main Authors: Schneider Werner Vianna, Thiago, Sartoretto, Suelen Cristina, Neves Novellino Alves, Adriana Terezinha, Figueiredo de Brito Resende, Rodrigo, de Almeida Barros Mourão, Carlos Fernando, de Albuquerque Calasans-Maia, Jose, Martinez-Zelaya, Victor R, Malta Rossi, Alexandre, Granjeiro, Jose Mauro, Calasans-Maia, Monica Diuana, Seabra Louro, Rafael
Format: Article
Language:English
Subjects:
bone regeneration
critical-size defect
nanostructured carbonated hydroxyapatite
rhBMP-2
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Summary:Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system's primary function is to hold these growth factors at the wound's site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups ( = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µCT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µCT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) ( < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb11040087