Development and validation of the quantitative determination procedure of iodine in the iodides form in the kelp thallus by the ionometry method

• Published in: Journal of pharmacy & bioallied science 2020-07, Vol.12 (3), p.277-283
• Main Authors: Nikulin, Alexander, Potanina, Olga, Okuneva, Marina, Abramovich, Rimma, Bokov, Dmitry, Smyslova, Olga
• Format: Article
• Language: English
• Subjects: Activation analysis; Electrodes; Iodine; iodine in the iodides form; kelp thallus; Laminaria; Neutron activation analysis; Original; pharmacopoeial analysis; Silver chloride; validation
• ISSN: 0975-7406; 0976-4879; 0975-7406
• DOI: 10.4103/jpbs.JPBS_198_20

Introduction: Iodine is an important compound in the kelp thallus; it should be determined to control the quality of crude herbal drugs of Laminaria sp. The ionometry method is perspective iodine (in the iodides form) determination method in the crude herbal drugs; it is characterized by the availability and relative cheapness of iodide-selective electrodes and equipment in general. This method provides an effective combination of the determination step with the fast, simple, and safe step of sample preparation. Aim: The current study aims to develop and validate a simple, effective procedure for the quantitative determination of iodine in the form of iodide by ionometry in the kelp thallus (Laminaria sp.). Materials and methods: The determination of iodides was carried out by using the "Ecotest-120" pH meter. "Ekom-I" was used as an ion-selective electrode. Silver chloride electrode "ESR 10101" was used as a reference electrode. Results and Discussion: The developed procedure has a suitable level of linearity (correlation coefficient = 0.9995%), correctness (variation coefficient = 1.58%), repeatability (variation coefficient = 6.67%), and analytical area (0.03-209.4 μg/mL analyte in the test solution). The procedure allows us to determine iodine in the form of iodides with an accuracy comparable to the accuracy of neutron activation analysis and can be recommended as an alternative to titrimetric methods existing in the world-leading pharmacopoeias.