Effect of a High-Fat Diet and Metformin on Placental mTOR Signaling in Mice

Abstract Objective  This study was aimed to measure the effects of a high-fat diet and metformin on placental mechanistic target of rapamycin (mTOR) signaling in mice. Study Design  Pregnant friend virus B (FVB)-strain mice were allocated on embryonic day (e) 0.5 to one of four groups; group 1: cont...

Full description

Saved in:
Bibliographic Details
Published in:American journal of perinatology reports 2019-04, Vol.9 (2), p.e138-e143
Main Authors: Grace, Matthew R., Dotters-Katz, Sarah K., Zhou, Chunxiao, Manuck, Tracy, Boggess, Kim, Bae-Jump, Victoria
Format: Article
Language:English
Subjects:
high-fat diet
metformin
mice
mtor
Original
Original Article
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Objective  This study was aimed to measure the effects of a high-fat diet and metformin on placental mechanistic target of rapamycin (mTOR) signaling in mice. Study Design  Pregnant friend virus B (FVB)-strain mice were allocated on embryonic day (e) 0.5 to one of four groups; group 1: control diet (CD, 10% fat) + control treatment (CT), group 2: CD + metformin treatment (MT), group 3: high-fat diet (HFD, 60% fat) + CT, and group 4: HFD + MT. Metformin (2.5 mg/mL) was provided in water; CT mice received water. Fetuses and placentas were collected. Western blot measured placental p-Akt and p-S6 expression. Results  20 dams (five/group) and 192 fetuses were studied. Compared with CD-fed, HFD-fed dams had higher placental p-Akt protein expression ( p  
ISSN:2157-6998
2157-7005
DOI:10.1055/s-0039-1683362