Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation

This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV)....

Full description

Saved in:
Bibliographic Details
Published in:Orbital : The Electronic Journal of Chemistry 2019-08, Vol.11 (4)
Main Authors: Alves, Thais Silva, Fiorucci, Antonio Rogério, Arruda, Gilberto José
Format: Article
Language:English
Subjects:
carbon paste electrode
determination
hormones
medications
voltammetry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 4
container_start_page
container_title Orbital : The Electronic Journal of Chemistry
container_volume 11
creator Alves, Thais Silva
Fiorucci, Antonio Rogério
Arruda, Gilberto José
description This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV). The investigation of Estradiol at carbon paste electrode revealed a non-reversible oxidation peak at +640 mV vs. Ag/AgCl, which was used for electrochemical detection of Estradiol. The operating parameters (pH, frequency, step potential, and amplitude) were optimized in relation to the peak current intensity, and a calibration curve was set up in a concentration range of 0.059 - 2.997 mg L-1, with a detection limit of 21.85 µg L-1. After calibration curve was plotted, the developed procedure was applied to determine Estradiol in parmacheutical formulation. These results show that the proposed method can be used for Estradiol quantification in pharmaceutical formulations with high sensitivity, specificity, stability, and reproducibility, and can be applied in analytical routines in laboratories of quality control analysis in drugs. DOI: http://dx.doi.org/10.17807/orbital.v11i4.1332
doi_str_mv 10.17807/orbital.v11i4.1332
format article
fullrecord <record><control><sourceid>doaj_cross</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_0a63c1ece9e1496d9ac8416b64753327</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_0a63c1ece9e1496d9ac8416b64753327</doaj_id><sourcerecordid>oai_doaj_org_article_0a63c1ece9e1496d9ac8416b64753327</sourcerecordid><originalsourceid>FETCH-LOGICAL-c310t-d96c968525b771edaa2c4e2e25d0552ec91283381d086db1157143ed6f0eb8ac3</originalsourceid><addsrcrecordid>eNpNkd1K5TAQgMuisKI-wd7kBXrMNG1-Lhd_VkHRhfU6TJOpJ5I2kuYIvtY-iM9krSLOzQwzk48hX1X9Ar4Bpbk6SbkPBePmGSC0GxCi-VEdgNFtLdtG732rf1bH8_zIlxBGKW0OqnxGzxTT00hTYWlgOLHzSK7k5LY0BoeR3VDZJp9ienhhQ8qsbIn93eFUwrDMS0jT-8PX_zXNJaMPKbIwsbst5hEd7coKuUh53MV1-6jaHzDOdPyZD6v7i_N_p5f19e2fq9Pf17UTwEvtjXRG6q7peqWAPGLjWmqo6TzvuoacgUYLocFzLX0P0CloBXk5cOo1OnFYXX1wfcJH-5TDiPnFJgx2baT8YDEv10WyHKVwQI4MQWukN-h0C7KXreqW71QLS3ywXE7znGn44gG3qwX7acGuFuy7BfEG6wOAAw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Alves, Thais Silva ; Fiorucci, Antonio Rogério ; Arruda, Gilberto José</creator><creatorcontrib>Alves, Thais Silva ; Fiorucci, Antonio Rogério ; Arruda, Gilberto José</creatorcontrib><description>This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV). The investigation of Estradiol at carbon paste electrode revealed a non-reversible oxidation peak at +640 mV vs. Ag/AgCl, which was used for electrochemical detection of Estradiol. The operating parameters (pH, frequency, step potential, and amplitude) were optimized in relation to the peak current intensity, and a calibration curve was set up in a concentration range of 0.059 - 2.997 mg L-1, with a detection limit of 21.85 µg L-1. After calibration curve was plotted, the developed procedure was applied to determine Estradiol in parmacheutical formulation. These results show that the proposed method can be used for Estradiol quantification in pharmaceutical formulations with high sensitivity, specificity, stability, and reproducibility, and can be applied in analytical routines in laboratories of quality control analysis in drugs. DOI: http://dx.doi.org/10.17807/orbital.v11i4.1332</description><identifier>ISSN: 1984-6428</identifier><identifier>EISSN: 1984-6428</identifier><identifier>DOI: 10.17807/orbital.v11i4.1332</identifier><language>eng</language><publisher>Universidade Federal de Mato Grosso do Sul</publisher><subject>carbon paste electrode ; determination ; hormones ; medications ; voltammetry</subject><ispartof>Orbital : The Electronic Journal of Chemistry, 2019-08, Vol.11 (4)</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Alves, Thais Silva</creatorcontrib><creatorcontrib>Fiorucci, Antonio Rogério</creatorcontrib><creatorcontrib>Arruda, Gilberto José</creatorcontrib><title>Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation</title><title>Orbital : The Electronic Journal of Chemistry</title><description>This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV). The investigation of Estradiol at carbon paste electrode revealed a non-reversible oxidation peak at +640 mV vs. Ag/AgCl, which was used for electrochemical detection of Estradiol. The operating parameters (pH, frequency, step potential, and amplitude) were optimized in relation to the peak current intensity, and a calibration curve was set up in a concentration range of 0.059 - 2.997 mg L-1, with a detection limit of 21.85 µg L-1. After calibration curve was plotted, the developed procedure was applied to determine Estradiol in parmacheutical formulation. These results show that the proposed method can be used for Estradiol quantification in pharmaceutical formulations with high sensitivity, specificity, stability, and reproducibility, and can be applied in analytical routines in laboratories of quality control analysis in drugs. DOI: http://dx.doi.org/10.17807/orbital.v11i4.1332</description><subject>carbon paste electrode</subject><subject>determination</subject><subject>hormones</subject><subject>medications</subject><subject>voltammetry</subject><issn>1984-6428</issn><issn>1984-6428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpNkd1K5TAQgMuisKI-wd7kBXrMNG1-Lhd_VkHRhfU6TJOpJ5I2kuYIvtY-iM9krSLOzQwzk48hX1X9Ar4Bpbk6SbkPBePmGSC0GxCi-VEdgNFtLdtG732rf1bH8_zIlxBGKW0OqnxGzxTT00hTYWlgOLHzSK7k5LY0BoeR3VDZJp9ienhhQ8qsbIn93eFUwrDMS0jT-8PX_zXNJaMPKbIwsbst5hEd7coKuUh53MV1-6jaHzDOdPyZD6v7i_N_p5f19e2fq9Pf17UTwEvtjXRG6q7peqWAPGLjWmqo6TzvuoacgUYLocFzLX0P0CloBXk5cOo1OnFYXX1wfcJH-5TDiPnFJgx2baT8YDEv10WyHKVwQI4MQWukN-h0C7KXreqW71QLS3ywXE7znGn44gG3qwX7acGuFuy7BfEG6wOAAw</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Alves, Thais Silva</creator><creator>Fiorucci, Antonio Rogério</creator><creator>Arruda, Gilberto José</creator><general>Universidade Federal de Mato Grosso do Sul</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope></search><sort><creationdate>20190801</creationdate><title>Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation</title><author>Alves, Thais Silva ; Fiorucci, Antonio Rogério ; Arruda, Gilberto José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-d96c968525b771edaa2c4e2e25d0552ec91283381d086db1157143ed6f0eb8ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>carbon paste electrode</topic><topic>determination</topic><topic>hormones</topic><topic>medications</topic><topic>voltammetry</topic><toplevel>online_resources</toplevel><creatorcontrib>Alves, Thais Silva</creatorcontrib><creatorcontrib>Fiorucci, Antonio Rogério</creatorcontrib><creatorcontrib>Arruda, Gilberto José</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Orbital : The Electronic Journal of Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alves, Thais Silva</au><au>Fiorucci, Antonio Rogério</au><au>Arruda, Gilberto José</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation</atitle><jtitle>Orbital : The Electronic Journal of Chemistry</jtitle><date>2019-08-01</date><risdate>2019</risdate><volume>11</volume><issue>4</issue><issn>1984-6428</issn><eissn>1984-6428</eissn><abstract>This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV). The investigation of Estradiol at carbon paste electrode revealed a non-reversible oxidation peak at +640 mV vs. Ag/AgCl, which was used for electrochemical detection of Estradiol. The operating parameters (pH, frequency, step potential, and amplitude) were optimized in relation to the peak current intensity, and a calibration curve was set up in a concentration range of 0.059 - 2.997 mg L-1, with a detection limit of 21.85 µg L-1. After calibration curve was plotted, the developed procedure was applied to determine Estradiol in parmacheutical formulation. These results show that the proposed method can be used for Estradiol quantification in pharmaceutical formulations with high sensitivity, specificity, stability, and reproducibility, and can be applied in analytical routines in laboratories of quality control analysis in drugs. DOI: http://dx.doi.org/10.17807/orbital.v11i4.1332</abstract><pub>Universidade Federal de Mato Grosso do Sul</pub><doi>10.17807/orbital.v11i4.1332</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1984-6428
ispartof Orbital : The Electronic Journal of Chemistry, 2019-08, Vol.11 (4)
issn 1984-6428
1984-6428
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_0a63c1ece9e1496d9ac8416b64753327
source EZB-FREE-00999 freely available EZB journals
subjects carbon paste electrode
determination
hormones
medications
voltammetry
title Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T04%3A00%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20an%20Electrochemical%20Methodology%20for%20the%20Quantification%20of%20%CE%B2-estradiol%20in%20Pharmaceutical%20Formulation&rft.jtitle=Orbital%20:%20The%20Electronic%20Journal%20of%20Chemistry&rft.au=Alves,%20Thais%20Silva&rft.date=2019-08-01&rft.volume=11&rft.issue=4&rft.issn=1984-6428&rft.eissn=1984-6428&rft_id=info:doi/10.17807/orbital.v11i4.1332&rft_dat=%3Cdoaj_cross%3Eoai_doaj_org_article_0a63c1ece9e1496d9ac8416b64753327%3C/doaj_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c310t-d96c968525b771edaa2c4e2e25d0552ec91283381d086db1157143ed6f0eb8ac3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true