The challenge of ovarian tissue culture: 2D versus 3D culture

Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with culture...

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Published in:Journal of ovarian research 2021-11, Vol.14 (1), p.147-147, Article 147
Main Authors: Pais, Ana Sofia, Reis, Sandra, Laranjo, Mafalda, Caramelo, Francisco, Silva, Fátima, Botelho, Maria Filomena, Almeida-Santos, Teresa
Format: Article
Language:English
Subjects:
Alginate
Alginic acid
Analysis
Apoptosis
Cancer
Cell culture
Cell proliferation
Cryopreservation
Cryopreservation - methods
Cytotoxicity
Edema
Female
Fertility
Fertility preservation
Fertility Preservation - methods
Follicles
Humans
Hydrogels
Infertility
Morphology
Ovarian Follicle - diagnostic imaging
Ovaries
Ovary
Recovery of function
Tissue culture
Tissue culture techniques
Tissue Culture Techniques - methods
Transplants & implants
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Summary:Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. To increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells, several studies have been carried out with culture of ovarian tissue. The aim of this study was to compare a conventional (2D) culture with an alginate matrix three-dimensional (3D) model for ovarian tissue culture. The ovarian tissue culture within the alginate matrix (3D) was similar to 2D culture, regarding follicular density and cell apoptosis in follicles and stroma. The proliferation rate remained stable in both models for follicles, but for stromal cell proliferation it decreased only in 3D culture (p = 0.001). At 24 h of culture, cytotoxicity was lower in the 3D model (p = 0.006). As culture time increased, cytotoxicity seemed similar. Degradation of the tissue was suggested by the histological score analysis of tissue morphology after 72 h of culture. Tissue injury was greater (p = 0.01) in 3D culture due to higher interstitial oedema (p = 0.017) and tissue necrosis (p = 0.035). According to our results, 3D culture of ovarian tissue has no advantage over 2Dculture; it is more time consuming and difficult to perform and has worse reproducibility.
ISSN:1757-2215
1757-2215
DOI:10.1186/s13048-021-00892-z