Differential Susceptibility and Innate Immune Response of Aedes aegypti and Aedes albopictus to the Haitian Strain of the Mayaro Virus

Mayaro (MAYV) is an emerging arthropod-borne virus belonging to the Alphavirus genus of the Togaviridae family. Although forest-dwelling Haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating Aedes ssp mosquitoes. Here we assess the susceptibil...

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Bibliographic Details
Published in:Viruses 2019-10, Vol.11 (10), p.924
Main Authors: Diop, Fodé, Alout, Haoues, Diagne, Cheikh Tidiane, Bengue, Michèle, Baronti, Cécile, Hamel, Rodolphe, Talignani, Loïc, Liegeois, Florian, Pompon, Julien, Vargas, Ronald E Morales, Nougairède, Antoine, Missé, Dorothée
Format: Article
Language:English
Subjects:
Aedes
Aedes aegypti
Aedes albopictus
Antimicrobial peptides
arbovirus
Cecropin
chondrocytes
Culicidae
Females
Fever
Gene expression
Gene regulation
Genotype & phenotype
Human health and pathology
Immune response
Immunology
Infections
Infectious diseases
Innate immunity
Life Sciences
mayaro virus
Midgut
ml26a
Mosquitoes
niemann–pick type c1
Npc1 protein
Serine
Transcription
Viruses
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Summary:Mayaro (MAYV) is an emerging arthropod-borne virus belonging to the Alphavirus genus of the Togaviridae family. Although forest-dwelling Haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating Aedes ssp mosquitoes. Here we assess the susceptibility of Aedes aegypti and Aedes albopictus to infection with MAYV and their innate immune response at an early stage of infection. Aedes albopictus was more susceptible to infection with MAYV than Ae. aegypti. Analysis of transcript levels of twenty immunity-related genes by real-time PCR in the midgut of both mosquitoes infected with MAYV revealed increased expression of several immune genes, including CLIP-domain serine proteases, the anti-microbial peptides defensin A, E, cecropin E, and the virus inducible gene. The regulation of certain genes appeared to be Aedes species-dependent. Infection of Ae. aegypti with MAYV resulted in increased levels of myeloid differentiation2-related lipid recognition protein (ML26A) transcripts, as compared to Ae. albopictus. Increased expression levels of thio-ester-containing protein 22 (TEP22) and Niemann–Pick type C1 (NPC1) gene transcripts were observed in infected Ae. albopictus, but not Ae. aegypti. The differences in these gene expression levels during MAYV infection could explain the variation in susceptibility observed in both mosquito species.
ISSN:1999-4915
1999-4915
DOI:10.3390/v11100924