Comparison of protein expression profiles of different stages of lymph nodes metastasis in breast cancer

Breast cancer is the most common cancer among women worldwide. Breast cancer metastasis primarily happens through lymphatic system, where the extent of lymph node metastasis is the major factor influencing staging, prognosis and therapeutic decision of the disease. We aimed to study the protein expr...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological sciences 2012-01, Vol.8 (3), p.353-362
Main Authors: Lee, Hui-Hua, Lim, Chu-Ai, Cheong, Yew-Teik, Singh, Manjit, Gam, Lay-Harn
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Blotting, Western
Breast - metabolism
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Carcinoma, Ductal, Breast - genetics
Carcinoma, Ductal, Breast - metabolism
Carcinoma, Ductal, Breast - secondary
Chromatography, High Pressure Liquid
Electrophoresis, Gel, Two-Dimensional
Female
Gene Expression Profiling
Humans
Lymphatic Metastasis - genetics
Middle Aged
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
Neoplasm Staging
Research Paper
Tandem Mass Spectrometry
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Breast cancer is the most common cancer among women worldwide. Breast cancer metastasis primarily happens through lymphatic system, where the extent of lymph node metastasis is the major factor influencing staging, prognosis and therapeutic decision of the disease. We aimed to study the protein expression changes in different N (regional lymph nodes) stages of breast cancer. Protein expression profiles of breast cancerous and adjacent normal tissues were mapped by proteomics approach that comprises of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and tandem mass spectrometry (LC-MS/MS) analysis. Calreticulin and tropomyosin alpha 3 chains were the common up-regulated proteins in N0, N1 and N2 stages of breast cancer. Potential biomarker for each N stage was HSP 70 for N0, 80 k protein H precursor and PDI for N1 stage while 78 kDa glucose-regulated protein was found useful for N2 stage. In addition, significant up-regulation of PDI A3 was detected only in the metastasized breast cancer. The up-regulation expression of these proteins in cancerous tissues can potentially use as indicators for diagnosis, treatment and prognosis of different N stages of breast cancer.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.3157