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Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways
Mastitis is an economically important disease in dairy cows, which is often caused by Staphylococcus aureus ( S. aureus ). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have al...
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| Published in: | Frontiers in veterinary science 2020-07, Vol.7, p.443-443 |
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| container_title | Frontiers in veterinary science |
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| creator | Liu, Kangjun Ding, Tao Fang, Li Cui, Luying Li, Jun Meng, Xia Zhu, Guoqiang Qian, Chen Wang, Heng Li, Jianji |
| description | Mastitis is an economically important disease in dairy cows, which is often caused by
Staphylococcus aureus
(
S. aureus
). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by
S. aureus
. The
S. aureus
-induced rat model was established and three doses (0.2, 2, 20 μg/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1β, TNF-α, and IL-6, were detected using qRT-PCR. The key proteins of NF-κB and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease
S. aureus
load in the tissues. The overexpression levels of IL-1β, TNF-α, and IL-6 induced by
S. aureus
were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-κB and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused by
S. aureus
and alleviate the inflammatory response. |
| doi_str_mv | 10.3389/fvets.2020.00443 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_0aa75f162c08478994590c9f46d97b46</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_0aa75f162c08478994590c9f46d97b46</doaj_id><sourcerecordid>2437848320</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-2f722dcd25b9f1264dbb6900de95d5250bcb27d973c404971de6eeee09748a053</originalsourceid><addsrcrecordid>eNpVksFuEzEQhlcIRKvQO0cfuWyZtb3r9QUpVLREFFoROFuztjdxtbGD7Q3KS_BAPATPxCapEJ3LjGb--eYwf1G8ruCSsVa-7Xc2p0sKFC4BOGfPinNKpSgr0cjn_9VnxUVKDwBQ1VywFl4WZ4y2dQW0OS9-3cUVeqfJ0g7Wu3FD5hs7uBAx20SWGbfr_RB00HpMBMdox1QuvBm1NeQzpuyyS8R58hVzIt2eLPzadVPTr0heWzLX2e0wu-BJ6MmX6_LP7_cE_bQ7v_9Elm7lcTho7zGvf-I-vSpe9Dgke_GYZ8X36w_frj6Wt3c3i6v5bak5k7mkvaDUaEPrTvYVbbjpukYCGCtrU9MaOt1RYaRgmgOXojK2sVOAFLxFqNmsWJy4JuCD2ka3wbhXAZ06NkJcKYzZ6cEqQBR1XzVUQ8tFKyWvJWjZ82bid7yZWO9OrO3YbazR1ueIwxPo04l3a7UKOyU4NM30uFnx5hEQw4_Rpqw2Lmk7DOhtGJOinImWt4zCJIWTVMeQUrT9vzMVqIMr1NEV6uAKdXQF-wvCRqyH</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2437848320</pqid></control><display><type>article</type><title>Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways</title><source>PMC (PubMed Central)</source><creator>Liu, Kangjun ; Ding, Tao ; Fang, Li ; Cui, Luying ; Li, Jun ; Meng, Xia ; Zhu, Guoqiang ; Qian, Chen ; Wang, Heng ; Li, Jianji</creator><creatorcontrib>Liu, Kangjun ; Ding, Tao ; Fang, Li ; Cui, Luying ; Li, Jun ; Meng, Xia ; Zhu, Guoqiang ; Qian, Chen ; Wang, Heng ; Li, Jianji</creatorcontrib><description>Mastitis is an economically important disease in dairy cows, which is often caused by
Staphylococcus aureus
(
S. aureus
). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by
S. aureus
. The
S. aureus
-induced rat model was established and three doses (0.2, 2, 20 μg/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1β, TNF-α, and IL-6, were detected using qRT-PCR. The key proteins of NF-κB and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease
S. aureus
load in the tissues. The overexpression levels of IL-1β, TNF-α, and IL-6 induced by
S. aureus
were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-κB and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused by
S. aureus
and alleviate the inflammatory response.</description><identifier>ISSN: 2297-1769</identifier><identifier>EISSN: 2297-1769</identifier><identifier>DOI: 10.3389/fvets.2020.00443</identifier><identifier>PMID: 32851026</identifier><language>eng</language><publisher>Frontiers Media S.A</publisher><subject>MAPK ; mastitis ; NF-κB ; organic selenium ; selenohomolanthionine ; Staphylococcus aureus ; Veterinary Science</subject><ispartof>Frontiers in veterinary science, 2020-07, Vol.7, p.443-443</ispartof><rights>Copyright © 2020 Liu, Ding, Fang, Cui, Li, Meng, Zhu, Qian, Wang and Li. 2020 Liu, Ding, Fang, Cui, Li, Meng, Zhu, Qian, Wang and Li</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-2f722dcd25b9f1264dbb6900de95d5250bcb27d973c404971de6eeee09748a053</citedby><cites>FETCH-LOGICAL-c439t-2f722dcd25b9f1264dbb6900de95d5250bcb27d973c404971de6eeee09748a053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406644/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406644/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids></links><search><creatorcontrib>Liu, Kangjun</creatorcontrib><creatorcontrib>Ding, Tao</creatorcontrib><creatorcontrib>Fang, Li</creatorcontrib><creatorcontrib>Cui, Luying</creatorcontrib><creatorcontrib>Li, Jun</creatorcontrib><creatorcontrib>Meng, Xia</creatorcontrib><creatorcontrib>Zhu, Guoqiang</creatorcontrib><creatorcontrib>Qian, Chen</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Li, Jianji</creatorcontrib><title>Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways</title><title>Frontiers in veterinary science</title><description>Mastitis is an economically important disease in dairy cows, which is often caused by
Staphylococcus aureus
(
S. aureus
). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by
S. aureus
. The
S. aureus
-induced rat model was established and three doses (0.2, 2, 20 μg/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1β, TNF-α, and IL-6, were detected using qRT-PCR. The key proteins of NF-κB and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease
S. aureus
load in the tissues. The overexpression levels of IL-1β, TNF-α, and IL-6 induced by
S. aureus
were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-κB and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused by
S. aureus
and alleviate the inflammatory response.</description><subject>MAPK</subject><subject>mastitis</subject><subject>NF-κB</subject><subject>organic selenium</subject><subject>selenohomolanthionine</subject><subject>Staphylococcus aureus</subject><subject>Veterinary Science</subject><issn>2297-1769</issn><issn>2297-1769</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVksFuEzEQhlcIRKvQO0cfuWyZtb3r9QUpVLREFFoROFuztjdxtbGD7Q3KS_BAPATPxCapEJ3LjGb--eYwf1G8ruCSsVa-7Xc2p0sKFC4BOGfPinNKpSgr0cjn_9VnxUVKDwBQ1VywFl4WZ4y2dQW0OS9-3cUVeqfJ0g7Wu3FD5hs7uBAx20SWGbfr_RB00HpMBMdox1QuvBm1NeQzpuyyS8R58hVzIt2eLPzadVPTr0heWzLX2e0wu-BJ6MmX6_LP7_cE_bQ7v_9Elm7lcTho7zGvf-I-vSpe9Dgke_GYZ8X36w_frj6Wt3c3i6v5bak5k7mkvaDUaEPrTvYVbbjpukYCGCtrU9MaOt1RYaRgmgOXojK2sVOAFLxFqNmsWJy4JuCD2ka3wbhXAZ06NkJcKYzZ6cEqQBR1XzVUQ8tFKyWvJWjZ82bid7yZWO9OrO3YbazR1ueIwxPo04l3a7UKOyU4NM30uFnx5hEQw4_Rpqw2Lmk7DOhtGJOinImWt4zCJIWTVMeQUrT9vzMVqIMr1NEV6uAKdXQF-wvCRqyH</recordid><startdate>20200730</startdate><enddate>20200730</enddate><creator>Liu, Kangjun</creator><creator>Ding, Tao</creator><creator>Fang, Li</creator><creator>Cui, Luying</creator><creator>Li, Jun</creator><creator>Meng, Xia</creator><creator>Zhu, Guoqiang</creator><creator>Qian, Chen</creator><creator>Wang, Heng</creator><creator>Li, Jianji</creator><general>Frontiers Media S.A</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20200730</creationdate><title>Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways</title><author>Liu, Kangjun ; Ding, Tao ; Fang, Li ; Cui, Luying ; Li, Jun ; Meng, Xia ; Zhu, Guoqiang ; Qian, Chen ; Wang, Heng ; Li, Jianji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-2f722dcd25b9f1264dbb6900de95d5250bcb27d973c404971de6eeee09748a053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>MAPK</topic><topic>mastitis</topic><topic>NF-κB</topic><topic>organic selenium</topic><topic>selenohomolanthionine</topic><topic>Staphylococcus aureus</topic><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Kangjun</creatorcontrib><creatorcontrib>Ding, Tao</creatorcontrib><creatorcontrib>Fang, Li</creatorcontrib><creatorcontrib>Cui, Luying</creatorcontrib><creatorcontrib>Li, Jun</creatorcontrib><creatorcontrib>Meng, Xia</creatorcontrib><creatorcontrib>Zhu, Guoqiang</creatorcontrib><creatorcontrib>Qian, Chen</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Li, Jianji</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Kangjun</au><au>Ding, Tao</au><au>Fang, Li</au><au>Cui, Luying</au><au>Li, Jun</au><au>Meng, Xia</au><au>Zhu, Guoqiang</au><au>Qian, Chen</au><au>Wang, Heng</au><au>Li, Jianji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways</atitle><jtitle>Frontiers in veterinary science</jtitle><date>2020-07-30</date><risdate>2020</risdate><volume>7</volume><spage>443</spage><epage>443</epage><pages>443-443</pages><issn>2297-1769</issn><eissn>2297-1769</eissn><abstract>Mastitis is an economically important disease in dairy cows, which is often caused by
Staphylococcus aureus
(
S. aureus
). Selenium is an indispensable element for physiological function and contributes to reduce injury of the mammary glands in mastitis. However, adequate sources of selenium have always been an important consideration for livestock. Therefore, the study aimed to explore the protective effect and mechanism of Selenohomolanthionine (SeHLan) on mastitis induced by
S. aureus
. The
S. aureus
-induced rat model was established and three doses (0.2, 2, 20 μg/kg body weight/day) of dietary OS were supplemented. The bacterial load, histopathology, and myeloperoxidase (MPO) of the mammary glands were performed and determined. Cytokines, including interleukin (IL)-1β, TNF-α, and IL-6, were detected using qRT-PCR. The key proteins of NF-κB and MAPK signaling pathways were analyzed by Western blot. The results revealed that OS supplementation could reduce the recruitment of neutrophils and macrophages in mammary tissues, but did not decrease
S. aureus
load in the tissues. The overexpression levels of IL-1β, TNF-α, and IL-6 induced by
S. aureus
were inhibited after OS treatment. Furthermore, the increased phosphorylation of NF-κB and MAPKs proteins were also suppressed. The results suggest that dietary supplementation with adequate OS during pregnancy contributes to protect the mammary glands from injury caused by
S. aureus
and alleviate the inflammatory response.</abstract><pub>Frontiers Media S.A</pub><pmid>32851026</pmid><doi>10.3389/fvets.2020.00443</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | MAPK mastitis NF-κB organic selenium selenohomolanthionine Staphylococcus aureus Veterinary Science |
| title | Organic Selenium Ameliorates Staphylococcus aureus-Induced Mastitis in Rats by Inhibiting the Activation of NF-κB and MAPK Signaling Pathways |
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