Postharvest UV-B Irradiation Stimulated Ginsenoside Rg1 Biosynthesis through Nitric Oxide (NO) and Jasmonic Acid (JA) in Panax quinquefolius Roots

• Published in: Molecules (Basel, Switzerland) Switzerland), 2019-04, Vol.24 (8), p.1462
• Main Authors: Zhou, Jie, Ran, Zhi-fang, Yang, Xiao-tong, Li, Jia
• Format: Article
• Language: English
• Subjects: Accumulation; Acids; Biosynthesis; Brief Report; Drying; Experiments; Inhibitors; Irradiation; Jasmonic acid; Metabolites; Nitric oxide; Nitric-oxide synthase; Panax quinquefolius; Physiology; postharvest UV-B radiation; Rg1; Roots; Signal transduction; Ultraviolet radiation
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24081462

The study highlights the influence and signal transduction mechanism of postharvest UV-B on the production of Rg1 in Panax quinquefolius roots during the drying process. The results showed that postharvest UV-B irradiation induced generation of nitric oxide (NO), jasmonic acid (JA), and ginsenoside Rg1 of P. quinquefolius roots. The UV-B-induced increase of Rg1 was suppressed by NO-specific scavenger (cPTIO) and NOS inhibitors (PBITU), JA synthesis inhibitor (SHAM), and JA synthesis inhibitor (PrGall), indicating that NO and JA played essential parts in UV-B-induced Rg1. External NO inhibitors treatment inhibited UV-B-induced accumulation of NO and JA, which suggested that NO was located upstream of the JA signal pathway. NO-caused Rg1 was inhibited by SHAM and PrGall, implying JA participated in transmitting signal NO to Rg1 accumulation. In other words, NO mediated the postharvest UV-B-induced Rg1 accumulation by the JA-dependent pathway in P. quinquefolius roots during the drying process, which helps us understand the underlying mechanisms involved in UV-B-induced Rg1 production and provides information helpful for P. quinquefolius production.