Remedy of contamination of multidrug resistant Salmonella and Escherichia coli from betel leaves (Piper betle) keeping them fresh for long time

The present study was carried out to identify the associated Salmonella and Escherichia coli in betel leaves (Piper betle), and to develop an effective method to remove those microbes. Betel leaves were collected from local and whole sale markets, and borouj (cultivation place). Salmonella and E. co...

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Bibliographic Details
Published in:Journal of advanced veterinary and animal research 2018-03, Vol.5 (1), p.73-80
Main Authors: Kamal, Tazrin, Nazir, K, Parvej, Md, Rahman, Md, Rahman, Marzia, Khan, Mohammad, Ansari, Wahedul, Ahamed, Md, Ahmed, Sultan, Hossen, Md, Panna, Sayedun
Format: Article
Language:English
Subjects:
Antibiotics
Bacteria
Betel leaves
Contamination
Escherichia coli
Salmonella
Treatment
Biochemical tests
Cefotaxime
Chloramphenicol
Chloromycetin
Ciprofloxacin
Contamination
Cultivation
Drug resistance
E coli
Escherichia coli
Health care
Laboratories
Leaves
Markets
Multidrug resistance
Multidrug resistant organisms
Pathogens
Piper betle
Polymerase chain reaction
rRNA 16S
Salmonella
Vegetables
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Summary:The present study was carried out to identify the associated Salmonella and Escherichia coli in betel leaves (Piper betle), and to develop an effective method to remove those microbes. Betel leaves were collected from local and whole sale markets, and borouj (cultivation place). Salmonella and E. coli were isolated and identified by cultural, morphological, and biochemical tests followed by confirmation by polymerase chain reaction (PCR) targeting the genus specific 16S rRNA genes. Antibiogram of the isolated bacteria was performed by disc diffusion method. Different concentrations of Salmosan-A Soln were used to remediate the contaminating bacteria keeping the quality of betel leaves for longer periods. Total Salmonella counts in the betel leaves were 3.9x105, 4.9x106, 3.5x1Q4, 1.1x103 and 1.5x103 CFU/mL, while E. coli counts were 5.5x107, 6.3x107, 4.4x105, 3.3x103 and 3.1x103 CFU/mL in the betel leaves collected from K.R. market, Kewatkhali Bazaar, whole sale market, borouj in Kushtia and borouj in Natore, respectively. Antibiogram study revealed that the isolated bacteria were sensitive to doxycyclline, ciprofloxacin, chloramphenicol and cefotaxime. Application of 0.3% Salmosan-A Soln was found to be the most effective and suitable, where
ISSN:2311-7710
2311-7710
DOI:10.5455/javar.2018.e250