Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction

To investigate the effect of transplantation of stem cells from the bone marrow mononuclear cells (BMMC) associated with 15d-PGJ2-loaded nanoparticles in a rat model of chronic MI. Chronic myocardial infarction (MI) was induced by the ligation of the left anterior descending artery in 40 male Wistar...

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Bibliographic Details
Published in:Journal of functional biomaterials 2022-05, Vol.13 (2), p.59
Main Authors: Uemura, Laercio, Baggio Simeoni, Rossana, Bispo Machado Júnior, Paulo André, Gavazzoni Blume, Gustavo, Kremer Gamba, Luize, Sgarbossa Tonial, Murilo, Baggio Simeoni, Paulo Ricardo, Stadler Tasca Ribeiro, Victoria, Silvestre, Rodrigo, de Carvalho, Katherine Athayde Teixeira, Napimoga, Marcelo Henrique, Cesar Francisco, Júlio, Guarita-Souza, Luiz Cesar
Format: Article
Language:English
Subjects:
15d-PGJ2
Angiogenesis
Animal models
anti-inflammatory
Antibodies
Autografts
Bone marrow
bone marrow mononuclear cells
Bone marrow transplantation
Bone surgery
Connexin 43
Coronary vessels
Echocardiography
Electrical junctions
Flow cytometry
Gap junctions
Heart attacks
Inflammation
Injection
Laboratory animals
Leukocytes (mononuclear)
Myocardial Infarction
Myocardium
Nanoparticles
Ostomy
rats
Statistical analysis
Stem cell transplantation
Stem cells
Surgery
Transplantation
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Summary:To investigate the effect of transplantation of stem cells from the bone marrow mononuclear cells (BMMC) associated with 15d-PGJ2-loaded nanoparticles in a rat model of chronic MI. Chronic myocardial infarction (MI) was induced by the ligation of the left anterior descending artery in 40 male Wistar rats. After surgery, we transplanted bone marrow associated with 15d-PGJ2-loaded nanoparticle by intramyocardial injection (10 cells/per injection) seven days post-MI. Myocardial infarction was confirmed by echocardiography, and histological analyses of infarct morphology, gap junctions, and angiogenesis were obtained. Our results from immunohistochemical analyses demonstrated the presence of angiogenesis identified in the transplanted region and that there was significant expression of connexin-43 gap junctions, showing a more effective electrical and mechanical integration of the host myocardium. This study suggests that the application of nanoparticle technology in the prevention and treatment of MI is an emerging field and can be a strategy for cardiac repair.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb13020059