Evaluation of the LIAISON XL Zika Capture IgM II for the Diagnosis of Zika Virus Infections

The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV; 74 samples), dengue (DENV; 10 samples), chikungunya (CHIK V; 11 samples), rubella (RUBV; 10 sam...

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Bibliographic Details
Published in:Viruses 2020-01, Vol.12 (1), p.69
Main Authors: Pérez-Olmeda, Mayte, Sánchez-Seco, María Paz, Vázquez, Ana, Balfagón, Pilar, de la Fuente, Jesús, Murillo, María Ángeles, Minguito, Teodora, de Ory, Fernando
Format: Article
Language:English
Subjects:
Antibodies, Viral - blood
Antigens
chemiluminescent immunoassay
Chikungunya virus
Dengue fever
dengue viruses
enzyme immunoassay
Enzyme-Linked Immunosorbent Assay
Epidemiology
False Positive Reactions
Humans
Immunofluorescence
Immunoglobulin M
Immunoglobulin M - blood
Infections
Measles
Parvoviruses
Rubella
Sensitivity and Specificity
Serologic Tests
Serology
Urine
Virus Diseases - blood
Virus Diseases - diagnosis
Virus Diseases - virology
Viruses
Zika virus
Zika Virus - immunology
Zika Virus - isolation & purification
Zika Virus Infection - blood
Zika Virus Infection - diagnosis
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Summary:The aim of this study is to evaluate the performance characteristics of the LIAISON XL Zika Capture IgM II. For this purpose we tested 128 samples obtained from recent infections caused by the Zika (ZIKV; 74 samples), dengue (DENV; 10 samples), chikungunya (CHIK V; 11 samples), rubella (RUBV; 10 samples) and measles (MeV; 10 samples) viruses, as well as human parvovirus B19 (HPVB19; 13 samples). The results of the assay under evaluation are compared with those obtained from an indirect immunofluorescence (IIF) assay, and the discrepancies are resolved by considering other laboratory results (PCR and a plaque-reduction neutralization test). The LIAISON showed excellent sensitivity (100%). The specificity (91.25%) was hampered by some false-positive results in recent dengue virus, chikungunya virus, measles virus and human parvovirus B19 infections. The method evaluated is adequate, but the low specificity makes it necessary to consider the clinical and epidemiological contexts of patients, as well as other laboratory results.
ISSN:1999-4915
1999-4915
DOI:10.3390/v12010069