In vitro Culture with Cytokines Provides a Tool to Assess the Effector Functions of ILC2s in Peripheral Blood in Asthma

Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma. The goal of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of asthma and allergy 2021-01, Vol.14, p.13-22
Main Authors: Drake, Li Y, Bartemes, Kathleen R, Bachman, Kay A, Hagan, John B, Kita, Hirohito
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Asthma
Cell culture
Cytokines
Enzyme-linked immunosorbent assay
Flow cytometry
il-13
il-25
il-33
il-5
Interleukin 13
Interleukin 2
Interleukin 5
Leukocytes (mononuclear)
Lymphoid cells
Mucosa
Original Research
Patients
Peripheral blood mononuclear cells
Population
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Group 2 innate lymphoid cells (ILC2s) play crucial roles in type 2 immunity and asthma development. While ILC2s are resident in mucosal tissues, they also circulate in peripheral blood. It remains controversial whether ILC2s are increased in the peripheral blood of patients with asthma. The goal of this project was to study the effector functions of ILC2s in peripheral blood samples by in vitro culture with cytokines. Peripheral blood mononuclear cells (PBMCs) were collected from 11 adult patients with mild asthma and 12 healthy control subjects. The number of peripheral blood ILC2s in PBMCs was analyzed by flow cytometry. PBMCs were cultured with IL-33 and IL-25 without any antigens, and the amounts of type 2 cytokines in cell-free supernatants were analyzed by ELISA. In selected experiments, production of cytokines by ILC2s was analyzed by intracellular cytokine staining and flow cytometry. In response to either IL-33 or IL-25 stimulation, PBMCs from patients with mild asthma produced larger amounts of IL-5 and IL-13 than PBMCs from healthy control subjects. However, ILC2 numbers or proportions were not significantly different between these two groups. Flow cytometric analysis confirmed production of IL-5 by ILCs when stimulated with IL-33. In vitro culture of PBMCs with a cocktail of cytokines, such as either IL-33 or IL-25 plus IL-2, may provide a valuable tool to assess the effector functions of ILC2s and may serve as a biomarker for human asthma.
ISSN:1178-6965
1178-6965
DOI:10.2147/JAA.S286695