Hepatotoxicity prevention in Acetaminophen-induced HepG2 cells by red betel (Piper crocatum Ruiz and Pav) extract from Indonesia via antioxidant, anti-inflammatory, and anti-necrotic

Acetaminophen (APAP) is a widely used analgesic, but it may cause liver injury (hepatotoxicity) via oxidative stress that induced by N-acetyl-p-benzoquinone imine (NAPQI) in long term usage or overdose. Multiple inflammatory mediators were also found to contribute for this effect. Many medicinal pla...

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Bibliographic Details
Published in:Heliyon 2021-01, Vol.7 (1), p.e05620, Article e05620
Main Authors: Ginting, Chrismis Novalinda, Lister, I Nyoman Ehrich, Girsang, Ermi, Widowati, Wahyu, Yusepany, Dewani Tediana, Azizah, Alya Mardhotillah, Kusuma, Hanna Sari Widya
Format: Article
Language:English
Subjects:
Acetaminophen
Biochemistry
Hepatoprotective
HepG2 cells
Immunology
Inflammation
Natural product
Pharmaceutical science
Red betel leaves extract
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Summary:Acetaminophen (APAP) is a widely used analgesic, but it may cause liver injury (hepatotoxicity) via oxidative stress that induced by N-acetyl-p-benzoquinone imine (NAPQI) in long term usage or overdose. Multiple inflammatory mediators were also found to contribute for this effect. Many medicinal plants was known for its antioxidant and anti-inflammatory activities and one of them is Red betel (Piper crocatum Ruiz and Pav) from Indonesia. In this study, the red betel leaves extract (RBLE) protective effect against APAP-induced HepG2 cells was determined. APAP-induced HepG2 as hepatotoxicity cell model was treated with RBLE at 25 and 100 μg/mL. Protective effects of RBLE toward hepatotoxicity were evaluated by several parameters: tumor necrosis factor-α (TNF-α) concentration, reactive oxygen species (ROS) level, live cells percentage, apoptotic cells percentage, necrotic cells percentage, death cells percentage, CYP2E1 and GPX gene expression. The RBLE treatments (both 25 and 100 μg/mL) increased CYP2E1 and GPX gene expression also live cells percentage, while decreased ROS level, TNF-α concentration, also the percentage of death and necrotic cells. Red Betel leaves ethanol extract has hepatoprotective effect via anti-inflammatory, anti-necrotic, and antioxidant potency in liver injury model. Red betel leaves extract; Acetaminophen; HepG2 cells; Hepatoprotective; Biochemistry; Immunology; Inflammation; Natural product; Pharmaceutical science
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2020.e05620