Modulation of the MOP Receptor (μ Opioid Receptor) by Imidazo[1,2- a ]imidazole-5,6-Diones: In Search of the Elucidation of the Mechanism of Action

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-05, Vol.27 (9), p.2930
• Main Authors: Straszak, Dominik, Siwek, Agata, Głuch-Lutwin, Monika, Mordyl, Barbara, Kołaczkowski, Marcin, Pietrzak, Aldona, Rahnama-Hezavah, Mansur, Drop, Bartłomiej, Matosiuk, Dariusz
• Format: Article
• Language: English
• Subjects: Addictions; Allosteric properties; allosterism; Analgesia; Analgesics; Analgesics, Opioid - therapeutic use; Animals; antagonism; Aromatic compounds; Arrestin; Carbonyl compounds; Carbonyls; Chronic pain; Cricetinae; Cricetulus; Drug abuse; Drug dosages; Enkephalin, Ala-MePhe-Gly; Enkephalins; Functional testing; G protein-coupled receptors; Humans; imidazo[1,2-a]imidazoles; Imidazole; Imidazoles - pharmacology; Ligands; MOP receptor; Morphine; Morphine - pharmacology; Narcotics; Nonsteroidal anti-inflammatory drugs; OP3 receptor; Opioid receptors (type mu); Opioids; Pain; Pain perception; Receptors, Opioid, mu - metabolism; Selectivity; Side effects; Signal transduction; Software; μ-opioid receptor ligands
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27092930

The μ-opioid receptors belong to the family of G protein-coupled receptors (GPCRs), and their activation triggers a cascade of intracellular relays with the final effect of analgesia. Classical agonists of this receptor, such as morphine, are the main targets in the treatment of both acute and chronic pain. However, the dangerous side effects, such as respiratory depression or addiction, significantly limit their widespread use. The allosteric centers of the receptors exhibit large structural diversity within particular types and even subtypes. Currently, a considerable interest is aroused by the modulation of μ-opioid receptors. The application of such a technique may result in a reduction in the dose or even discontinuation of classical opiates, thus eliminating the side effects typical of this class of drugs. Our aim is to obtain a series of 1-aryl-5,6(1 )dioxo-2,3-dihydroimidazo[1,2- ]imidazole derivatives and provide more information about their activity and selectivity on OP3 (MOP, human mu opioid receptor). The study was based on an observation that some carbonyl derivatives of 1-aryl-2-aminoimidazoline cooperate strongly with morphine or DAMGO in sub-threshold doses, producing similar results to those of normal active doses. To elucidate the possible mechanism of such enhancement, we performed a few in vitro functional tests (involving cAMP and β-arrestin recruitment) and a radioligand binding assay on CHO-K1 cells with the expression of the OP3 receptor. One of the compounds had no orthosteric affinity or intrinsic activity, but inhibited the efficiency of DAMGO. These results allow to conclude that this compound is a negative allosteric modulator (NAM) of the human μ-opioid receptor.