Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics

• Published in: Foods 2023-04, Vol.12 (8), p.1605
• Main Authors: Wang, Zhaobin, Wang, Xiaoting, Sheng, Xiaowei, Zhao, Luling, Qian, Jing, Zhang, Jianhao, Wang, Jin
• Format: Article
• Language: English
• Subjects: Acids; Amino acids; antibacterial activity; Antibacterial agents; Bacteria; Beef; Biotechnology; Dehydrogenase; Disinfection; Efficiency; Electron microscopy; Food science; Glutathione; Gram-positive bacteria; Intracellular; Iodides; Lactic acid; Malate dehydrogenase; mechanism; Membrane permeability; Metabolic pathways; Metabolomics; Oxidative stress; Permeability; Plasma; Plasma jets; plasma-activated lactic acid; Propidium iodide; Proteins; Pseudomonas; Pseudomonas lundensis; Reactive oxygen species; Reduction; Scanning electron microscopy; Spoilage; Temperature; Transmission electron microscopy; untargeted metabolomics
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12081605

Plasma-activated liquid is a novel non-thermal antibacterial agent against a wide spectrum of foodborne bacteria, yet fewer studies focused on its disinfection of meat spoilage bacteria. In this study, the antibacterial properties of plasma-activated lactic acid (PALA) on isolated and identified from spoilage beef, were investigated. A plasma jet was used to treat lactic acid (0.05-0.20%) for 60-120 s. The results presented that the 0.2% LA solution treated with plasma for 120 s caused a 5.64 log reduction. Additionally, the surface morphology, membrane integrity and permeability were altered slightly and verified by scanning electron microscopy, double staining of SYTO-9 and propidium iodide, and a K test kit. The intracellular organization of the cells, observed by transmission electron microscopy, was damaged significantly. Increased intracellular reactive oxygen species (ROS) levels exceeded the antioxidant ability of glutathione (GSH), leading to a reduction in the activity of malate dehydrogenase (MDH), succinic dehydrogenase (SDH) and intracellular ATP levels. Metabolomics analysis indicated that the energy and synthesis of essential components, such as DNA and amino acid-related metabolic pathways, were disturbed. In conclusion, this research established a theoretical basis for the use of PALA in refrigerated beef preservation by shedding light on the bacteriostatic effect of PALA against .