Unveiling the Cutting-Edge Impact of Polarized Macrophage-Derived Extracellular Vesicles and MiRNA Signatures on TGF-β Regulation within Lung Fibroblasts

Depending on local cues, macrophages can polarize into classically activated (M1) or alternatively activated (M2) phenotypes. This study investigates the impact of polarized macrophage-derived Extracellular Vesicles (EVs) (M1 and M2) and their cargo of miRNA-19a-3p and miRNA-425-5p on TGF-β producti...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-07, Vol.25 (13), p.7490
Main Authors: Casara, Alvise, Conti, Maria, Bernardinello, Nicol, Tinè, Mariaenrica, Baraldo, Simonetta, Turato, Graziella, Semenzato, Umberto, Celi, Alessandro, Spagnolo, Paolo, Saetta, Marina, Cosio, Manuel G, Neri, Tommaso, Biondini, Davide, Bazzan, Erica
Format: Article
Language:English
Subjects:
Cells, Cultured
Communication
Cytokines
Dimensional analysis
EVs
Extracellular matrix
Extracellular vesicles
Extracellular Vesicles - genetics
Extracellular Vesicles - metabolism
Fibroblasts
Fibroblasts - metabolism
Flow cytometry
Gene Expression Regulation
Humans
Investigations
Lung - cytology
Lung - metabolism
Macrophage Activation - genetics
macrophages
Macrophages - metabolism
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNAs
Physiology
TGF-β
Transforming Growth Factor beta - metabolism
Wound healing
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Summary:Depending on local cues, macrophages can polarize into classically activated (M1) or alternatively activated (M2) phenotypes. This study investigates the impact of polarized macrophage-derived Extracellular Vesicles (EVs) (M1 and M2) and their cargo of miRNA-19a-3p and miRNA-425-5p on TGF-β production in lung fibroblasts. EVs were isolated from supernatants of M0, M1, and M2 macrophages and quantified using nanoscale flow cytometry prior to fibroblast stimulation. The concentration of TGF-β in fibroblast supernatants was measured using ELISA assays. The expression levels of miRNA-19a-3p and miRNA-425-5p were assessed via TaqMan-qPCR. TGF-β production after stimulation with M0-derived EVs and with M1-derived EVs increased significantly compared to untreated fibroblasts. miRNA-425-5p, but not miRNA-19a-3p, was significantly upregulated in M2-derived EVs compared to M0- and M1-derived EVs. This study demonstrates that EVs derived from both M0 and M1 polarized macrophages induce the production of TGF-β in fibroblasts, with potential regulation by miRNA-425-5p.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25137490