Gracillin Isolated from Reineckia carnea Induces Apoptosis of A549 Cells via the Mitochondrial Pathway

• Published in: Drug design, development and therapy development and therapy, 2021-01, Vol.15, p.233-243
• Main Authors: Yang, Jianqiong, Cao, Ling, Li, Yamei, Liu, Hai, Zhang, Minhong, Ma, Huamou, Wang, Biao, Yuan, Xiaoliang, Liu, Qian
• Format: Article
• Language: English
• Subjects: a549 cells; Analysis; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; antiproliferative activity; Apoptosis; Apoptosis - drug effects; B cells; Bcl-2 protein; Calcium; Calcium (intracellular); Calcium (mitochondrial); Caspase-3; Cell cycle; Cell growth; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Cough; Cytochrome; Cytochrome c; Cytochromes; Drug Screening Assays, Antitumor; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Ethanol; Flow cytometry; gracillin; Herbal medicine; High-performance liquid chromatography; Humans; Intracellular; Liliaceae - chemistry; Liquid chromatography; Lung cancer; Membrane potential; Membranes; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; NMR; Nuclear magnetic resonance; Original Research; Penicillin; Proteins; reineckia carnea; S phase; Saponins; Silica; Silica gel; Silicon dioxide; Spirostans - chemistry; Spirostans - isolation & purification; Spirostans - pharmacology; Steroids; Time dependence
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/DDDT.S278975

is commonly used to treat cough, pneumonia and other diseases in China. In our previous study, it was found that the ethanol extracts of have a strong inhibitory effect on the proliferation of human lung cancer A549 cells. Here, we isolated gracillin from ethanol extracts for the first time. Clarify the antiproliferation effect of gracillin on A549 cells and further explore its mechanisms via the mitochondrial pathway. Gracillin was isolated and purified by silica gel, D-101 macroporous resin and preparative RP-HPLC, then identified by NMR and HR-MS. The inhibitory effects of gracillin on the proliferation of A549 cells were detected by the MTS method. Its mechanisms were further explored by flow cytometry and Western blot. A steroid saponin, gracillin, was isolated and identified from for the first time. In a concentration-dependent and time-dependent manner, gracillin significantly inhibited the proliferation of A549 cells with an IC value at 2.54 μmol/L and induced morphological changes. The results of flow cytometry analysis showed that the apoptosis rate of A549 cells was significantly increased ( < 0.05), and the cells proportion was obviously arrested in S phase. The concentration of intracellular calcium was raised ( < 0.01), and the mitochondrial membrane potential was greatly decreased ( < 0.01). In addition, the expression levels of Bax, caspase-3, cleaved caspase-3, and cytochrome C were dramatically up-regulated while Bcl-2 was down-regulated ( < 0.05) in A549 cells. This study confirmed that gracillin has a significant antiproliferative effect on A549 cells. Gracillin could induce the apoptosis of A549 cells through the mitochondrial pathway, which might be associated with regulation of the concentration of intracellular calcium, the mitochondrial membrane potential and the expression levels of Bax, Bcl-2, caspase-3, cleaved caspase-3, and cytochrome C.