Anti-Biofilm Coatings Based on Chitosan and Lysozyme Functionalized Magnetite Nanoparticles

Biofilms represent a common and increasingly challenging problem in healthcare practices worldwide, producing persistent and difficult to manage infections. Researchers have started developing antibiotic-free treatment alternatives in order to decrease the risk of resistant microbial strain selectio...

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Bibliographic Details
Published in:Antibiotics (Basel) 2021-10, Vol.10 (10), p.1269
Main Authors: Spirescu, Vera Alexandra, Niculescu, Adelina-Gabriela, Slave, Ștefan, Bîrcă, Alexandra Cătalina, Dorcioman, Gabriela, Grumezescu, Valentina, Holban, Alina Maria, Oprea, Ovidiu-Cristian, Vasile, Bogdan Ștefan, Grumezescu, Alexandru Mihai, Nica, Ionela Cristina, Stan, Miruna Silvia, Andronescu, Ecaterina
Format: Article
Language:English
Subjects:
antibiofilm activity
Antibiotics
Antiinfectives and antibacterials
Antimicrobial agents
antimicrobial properties
Biocompatibility
Biofilms
Biomedical materials
Cell viability
Chitosan
Coatings
Cytotoxicity
Diploids
Drug resistance
Evaporation
Fabrication
Hospital costs
Infections
Investigations
Iron oxides
laser processing
Lasers
Lysozyme
Magnetite
magnetite-based coatings
Medical equipment
Microorganisms
Microscopy
Nanomaterials
Nanoparticles
nanostructured bioactive coatings
Physicochemical properties
Pulsed lasers
Spectrum analysis
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Summary:Biofilms represent a common and increasingly challenging problem in healthcare practices worldwide, producing persistent and difficult to manage infections. Researchers have started developing antibiotic-free treatment alternatives in order to decrease the risk of resistant microbial strain selection and for the efficient management of antibiotic tolerant biofilm infections. The present study reports the fabrication and characterization of magnetite-based nanostructured coatings for producing biofilm-resistant surfaces. Specifically, magnetite nanoparticles (Fe O ) were functionalized with chitosan (CS) and were blended with lysozyme (LyZ) and were deposited using the matrix-assisted pulsed laser evaporation (MAPLE) technique. A variety of characterization techniques were employed to investigate the physicochemical properties of both nanoparticles and nanocoatings. The biological characterization of the coatings assessed through cell viability and antimicrobial tests showed biocompatibility on osteoblasts as well as antiadhesive and antibiofilm activity against both Gram-negative and Gram-positive bacterial strains and no cytotoxic effect against human-cultured diploid cells.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics10101269