The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field

Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressin...

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Bibliographic Details
Published in:Viruses 2021-04, Vol.13 (4), p.606
Main Authors: Wang, Lidong, Zhang, Yanyan, Chen, Teng, Mi, Lijuan, Sun, Xuefei, Zhou, Xintao, Miao, Faming, Zhang, Shoufeng, Liu, Ye, Hu, Rongliang
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Baculoviridae - genetics
Capsid - immunology
Capsid - metabolism
Capsid protein
Capsid Proteins - genetics
Capsid Proteins - immunology
capsid subunit
China
Circoviridae Infections - immunology
Circoviridae Infections - prevention & control
Circoviridae Infections - veterinary
Circovirus - genetics
Circovirus - immunology
Cloning
Diarrhea
Diarrhea - prevention & control
Diarrhea - virology
E coli
Farms
Female
field vaccination
Infections
Male
Mink - virology
mink circovirus
Monoclonal antibodies
Plasmids
Proteins
recombinant baculovirus
Vaccination
Vaccines
Vaccines, Subunit - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Viruses
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Summary:Mink refractory diarrhea is a seasonal disease that occurs in many mink farms in China. Mink circovirus (MiCV) has been recognized as the causative agent of the disease. The aim of the study was to develop a subunit vaccine against mink refractory diarrhea. A recombinant baculovirus strain expressing the capsid protein was constructed using the baculovirus expression vector system (BEVS). A subunit vaccine was developed based on the capsid protein with appropriate adjuvant. Then, a field trial was carried out in two districts in order to evaluate the efficiency of the subunit vaccine. The field trial indicated that in total, only 1.8% of the minks developed typical diarrhea in the vaccinated group compared with 74.5% in the control group. The vaccination could significantly reduce the infection rate of MiCV among the mink herds and could restrain the virus' shedding from feces. Furthermore, the vaccinated group had a higher average litter size in the following year compared to the control group. Collectively, the results indicated that the subunit vaccine based on the capsid protein can provide reliable protection against MiCV infection.
ISSN:1999-4915
1999-4915
DOI:10.3390/v13040606