Detection of white sturgeon iridovirus (WSIV) in wild sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) in Poland

White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions, . high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for q...

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Bibliographic Details
Published in:Journal of veterinary research 2020-09, Vol.64 (3), p.363-368
Main Authors: Hofsoe-Oppermann, Paulina, Kiełpińska, Jolanta, Panicz, Remigiusz, Bergmann, Sven M.
Format: Article
Language:English
Subjects:
acipenser gueldenstaedtii
acipenser oxyrinchus
Deoxyribonucleic acid
disease control
DNA
Environmental conditions
freshwater aquaculture
Gills
Hybridization
Intestine
Kidneys
Lagoons
Polymerase chain reaction
Spleen
Sturgeon
vectors
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Summary:White sturgeon iridovirus (WSIV) disease is caused by a virus of the eponymous family and is mostly triggered by stressful environmental conditions, . high rearing density, excessive handling, or temporary loss of water. The aim of this study was to develop the most effective diagnostic method for quick and efficient confirmation or exclusion of the presence of WSIV. A total of 42 samples (spleen, gills, intestine, skin, kidney, and brain) were collected from eight sturgeon ( and ) aged ≤5+ farmed or caught between 2010 and 2014 in open waters (Dąbie Lake and Szczecin Lagoon). They were tested for WSIV presence using conventional PCR, qPCR, and hybridisation (ISH). In gross examination, all fish appeared to be healthy. Neither species showed clinical signs typical of WSIV infection. In the majority of cases, fragments of iridoviral DNA were found using molecular methods in the kidneys, and also in the liver, gills, and skin. The detection rate using ISH was 47.37% and most commonly the brain and kidney tissues were positive. The most efficient of the methods used was real-time PCR, with 100% effectiveness in detection of WSIV DNA. The study demonstrates the capabilities for WSIV diagnosis available to sturgeon farmers and water administrators, indicating useful methods of adequate sensitivity as well as organs to sample in order to achieve the highest probability of viral detection.
ISSN:2450-7393
2450-8608
2450-8608
DOI:10.2478/jvetres-2020-0055