Low molecular weight chitosan-insulin polyelectrolyte complex: characterization and stability studies

The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at...

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Bibliographic Details
Published in:Marine drugs 2015-03, Vol.13 (4), p.1765-1784
Main Authors: Al-Kurdi, Zakieh I, Chowdhry, Babur Z, Leharne, Stephen A, Al Omari, Mahmoud M H, Badwan, Adnan A
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Biological Availability
Biotransformation
Chitin - analogs & derivatives
Chitin - chemistry
Diabetes Mellitus, Experimental - blood
Diabetes Mellitus, Experimental - drug therapy
Diabetes Mellitus, Experimental - metabolism
Drug Delivery Systems
Drug Liberation
Drug Stability
Drug Storage
Electrolytes - chemistry
Excipients - chemistry
Hyperglycemia - prevention & control
Hypoglycemic Agents - administration & dosage
Hypoglycemic Agents - blood
Hypoglycemic Agents - pharmacokinetics
Hypoglycemic Agents - therapeutic use
insulin
Insulin - administration & dosage
Insulin - analogs & derivatives
Insulin - analysis
Insulin - blood
Insulin - pharmacokinetics
Insulin - therapeutic use
low molecular weight chitosan
Male
Marine
Micelles
Molecular Weight
oligochitosan
Particle Size
polyelectrolyte complex
Random Allocation
Rats, Sprague-Dawley
stability
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Summary:The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity.
ISSN:1660-3397
1660-3397
DOI:10.3390/md13041765