Coinfection of Two Rickettsia Species in a Single Tick Species Provides New Insight into Rickettsia - Rickettsia and Rickettsia -Vector Interactions

• Published in: Microbiology spectrum 2022-10, Vol.10 (5), p.e0232322-e0232322
• Main Authors: Pan, Yu-Sheng, Cui, Xiao-Ming, Du, Li-Feng, Xia, Luo-Yuan, Du, Chun-Hong, Bell-Sakyi, Lesley, Zhang, Ming-Zhu, Zhu, Dai-Yun, Dong, Yi, Wei, Wei, Zhao, Lin, Sun, Yi, Lv, Qing-Yu, Ye, Run-Ze, He, Zhi-Hai, Wang, Qian, Li, Liang-Jing, Yao, Ming-Guo, Xiong, Tao, Jiang, Jia-Fu, Cao, Wu-Chun, Jia, Na
• Format: Article
• Language: English
• Subjects: Actins; Animals; Bacteriology; Coinfection; competition; Haemaphysalis montgomeryi; Humans; interference; Ixodidae - microbiology; Research Article; Rickettsia - genetics; rickettsiae; tick; Ticks - microbiology
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.02323-22

Rickettsiae are obligate intracellular bacteria that can cause life-threatening illnesses. There is an ongoing debate as to whether established infections by one species preclude the maintenance of the second species in ticks. Here, we identified two species in inoculum from Haemaphysalis montgomeryi ticks and subsequently obtained pure isolates of each species by plaque selection. The two isolates were classified as a transitional group and spotted fever group rickettsiae and named Rickettsia hoogstraalii str CS and Rickettsia rhipicephalii str EH, respectively. The coinfection of these two species was detected in 25.6% of individual field-collected H. montgomeryi. In cell culture infection models, R. hoogstraalii str CS overwhelmed R. rhipicephalii str EH with more obvious cytopathic effects, faster plaque formation, and increased cellular growth when cocultured, and R. hoogstraalii str CS seemed to polymerize actin tails differently from R. rhipicephalii str EH . This work provides a model to investigate the mechanisms of both - and -vector interactions. The rickettsiae are a group of obligate intracellular Gram-negative bacteria that include human pathogens causing an array of clinical symptoms and even death. There is an important question in the field, that is whether one infection can block the superinfection of other rickettsiae. This work demonstrated the coinfection of two species in individual ticks and further highlighted that testing the rickettsial competitive exclusion hypothesis will undoubtedly be a promising area as methods for bioengineering and pathogen biocontrol become amenable for rickettsiae.