Sprague Dawley Rats Show More Severe Bone Loss, Osteophytosis and Inflammation Compared toWistar Han Rats in a High-Fat, High-Sucrose Diet Model of Joint Damage

In animal models, joint degeneration observed in response to obesogenic diet varies in nature and severity. In this study, we compare joint damage in Sprague Dawley and Wistar-Han rats in response to a high-fat, high-sucrose (HFS) diet groove model of osteoarthritis (OA). Wistar Han ( = 5) and Sprag...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-03, Vol.23 (7), p.3725
Main Authors: Warmink, Kelly, Rios, Jaqueline L, van Valkengoed, Devin R, Korthagen, Nicoline M, Weinans, Harrie
Format: Article
Language:English
Subjects:
animal models
Animals
Biomarkers
Diet, High-Fat - adverse effects
Disease Models, Animal
Inflammation
obesity
Obesity - metabolism
osteoarthritis
Osteoarthritis - diagnostic imaging
Osteoarthritis - etiology
Osteoarthritis - pathology
osteoporosis
Rats
Rats, Sprague-Dawley
Rats, Wistar
Sucrose - adverse effects
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Summary:In animal models, joint degeneration observed in response to obesogenic diet varies in nature and severity. In this study, we compare joint damage in Sprague Dawley and Wistar-Han rats in response to a high-fat, high-sucrose (HFS) diet groove model of osteoarthritis (OA). Wistar Han ( = 5) and Sprague Dawley ( = 5) rats were fed an HFS diet for 24 weeks. OA was induced 12 weeks after the diet onset by groove surgery in the right knee joint. The left knee served as a control. Outcomes were OARSI histopathology scoring, bone changes by µCT imaging, local (synovial and fat pad) and systemic (blood cytokine) inflammation markers. In both rat strains, the HFS diet resulted in a similar change in metabolic parameters, but only Sprague Dawley rats showed a large, osteoporosis-like decrease in trabecular bone volume. Osteophyte count and local joint inflammation were higher in Sprague Dawley rats. In contrast, cartilage degeneration and systemic inflammatory marker levels were similar between the rat strains. The difference in bone volume loss, osteophytosis and local inflammation suggest that both rat strains show a different joint damage phenotype and could, therefore, potentially represent different OA phenotypes observed in humans.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23073725