Characterization of the Bioactivity and Mechanism of Bactenecin Derivatives Against Food-Pathogens

• Published in: Frontiers in microbiology 2019-11, Vol.10, p.2593-2593
• Main Authors: Sun, Changbao, Gu, Liya, Hussain, Muhammad Altaf, Chen, Lijun, Lin, Li, Wang, Haimei, Pang, Shiyue, Jiang, Chenggang, Jiang, Zhanmei, Hou, Juncai
• Format: Article
• Language: English
• Subjects: antibacterial peptides; bactenecin derivatives; bioactivity; food-pathogens; mechanism; Microbiology
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.02593

With the emergence of multidrug-resistant bacteria, antimicrobial peptides (AMPs) are regarded as potential alternatives to traditional antibiotics or chemicals. We designed and synthesized six derivatives of bactenecin (L 2 C 3 V 10 C 11 , RLCRIVVIRVCR), including R 2 F 3 W 10 L 11 (RRFRIVVIRWLR), R 2 W 3 W 10 R 11 (RRWRIVVIRWRR), K 2 W 3 V 10 R 11 (RKWRIVVIRVRR), W 2 R 3 V 10 R 11 (RWRRIVVIRVRR), W 2 K 3 K 10 R 11 (RWKRIVVIRKRR), and K 2 R 3 R 10 K 11 (RKRRIVVIRRKR), by amino acid substitution to increase the net charge and reduce hydrophobicity gradually. The bioactivity and mechanisms of action of the designed peptides were investigated. The results indicated that the antimicrobial activity of the designed peptides was higher than that of bactenecin. The hemolytic activity and cytotoxicity of the designed peptides were significantly lower than those of bactenecin. The designed peptides exhibited a wide range of antimicrobial activity against food-pathogens, particularly peptides K 2 W 3 V 10 R 11 and W 2 R 3 V 10 R 11 ; in addition, the activity was maintained under physiological salt and heat conditions. Mechanism studies indicated that AMPs interacted with negatively charged bacterial cell membranes, resulting in the destruction of cell membrane integrity by increasing membrane permeability and changing transmembrane potential, leading to cell death. The present study suggested that peptides K 2 W 3 V 10 R 11 and W 2 R 3 V 10 R 11 exhibited potential as alternatives to traditional antibiotics or chemicals for the treatment of food-pathogens. These findings lead to the development of a potential method for the design of novel AMPs.