An 'activator-repressor' loop controls the anthocyanin biosynthesis in red-skinned pear

The color of red-skinned pear (Pyrus spp.) is primarily attributed to accumulation of anthocyanins, which provide nutritional benefits for human health and are closely associated with the commercial value of fruits. Here, we reported the functional characterization of a R2R3-MYB repressor PyMYB107,...

Full description

Saved in:
Bibliographic Details
Published in:Molecular horticulture 2024-07, Vol.4 (1), p.26-16, Article 26
Main Authors: Yang, Guangyan, Xue, Zhaolong, Lin-Wang, Kui, Chen, Guosong, Zhao, Yongqi, Chang, Yaojun, Xu, Shaozhuo, Sun, Manyi, Xue, Cheng, Li, Jiaming, Allan, Andrew C, Espley, Richard V, Wu, Jun
Format: Article
Language:English
Subjects:
Anthocyanin
Biosynthesis
Competition
Fruits
Genes
Localization
Mutation
MYB
Pear
Phylogenetics
Proteins
Repressor
Transcription factors
Transcriptional regulation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The color of red-skinned pear (Pyrus spp.) is primarily attributed to accumulation of anthocyanins, which provide nutritional benefits for human health and are closely associated with the commercial value of fruits. Here, we reported the functional characterization of a R2R3-MYB repressor PyMYB107, which forms an 'activator-repressor' loop to control anthocyanin accumulation in the red-skinned pear. PyMYB107 overexpression inhibited anthocyanin biosynthesis in both pear calli and fruits, while virus-induced gene silencing of PyMYB107 increased anthocyanin accumulation in pear fruits. Furthermore, ectopic expression of PyMYB107 decreased anthocyanin accumulation in tomato, strawberry and tobacco. PyMYB107 can competitively bind to PybHLH3 with PyMYB10/MYB114, thereby suppressing the transcriptional activation of key anthocyanin biosynthesis genes, PyANS and PyUFGT. Site-directed mutagenesis showed that mutations within the R3 domain and EAR motif of PyMYB107 eliminated its repressive activity. Additionally, PyMYB107 exhibited a comparable expression pattern to PyMYB10/MYB114 and was transcriptionally activated by them. Our finding advanced comprehension of the repression mechanism underlying anthocyanin accumulation, providing valuable molecular insights into improving quality of pear fruits.
ISSN:2730-9401
2730-9401
DOI:10.1186/s43897-024-00102-6