PACAP Promotes Matrix-Driven Adhesion of Cultured Adult Murine Neural Progenitors

New neurons are born throughout the life of mammals in germinal zones of the brain known as neurogenic niches: the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus. These niches contain a subpopulation of cells known as adult neural proge...

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Bibliographic Details
Published in:ASN neuro 2017-05, Vol.9 (3), p.1759091417708720-1759091417708720
Main Authors: Waschek, James A., Cohen, Joseph R., Chi, Gloria C., Proszynski, Tomasz J., Niewiadomski, Pawel
Format: Article
Language:English
Subjects:
Adult Stem Cells - cytology
Adult Stem Cells - metabolism
Animals
Cell Adhesion - physiology
Cells, Cultured
Culture Media, Conditioned
Cyclic AMP-Dependent Protein Kinases - metabolism
Extracellular Matrix - metabolism
Male
Mice, Inbred C57BL
Mice, Knockout
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neurogenesis - physiology
Neurons - cytology
Neurons - metabolism
Original Paper
Pituitary Adenylate Cyclase-Activating Polypeptide - metabolism
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - deficiency
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - genetics
Receptors, Vasoactive Intestinal Peptide - metabolism
Stem Cell Niche - physiology
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Summary:New neurons are born throughout the life of mammals in germinal zones of the brain known as neurogenic niches: the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus. These niches contain a subpopulation of cells known as adult neural progenitor cells (aNPCs), which self-renew and give rise to new neurons and glia. aNPCs are regulated by many factors present in the niche, including the extracellular matrix (ECM). We show that the neuropeptide PACAP (pituitary adenylate cyclase-activating polypeptide) affects subventricular zone-derived aNPCs by increasing their surface adhesion. Gene array and reconstitution assays indicate that this effect can be attributed to the regulation of ECM components and ECM-modifying enzymes in aNPCs by PACAP. Our work suggests that PACAP regulates a bidirectional interaction between the aNPCs and their niche: PACAP modifies ECM production and remodeling, in turn the ECM regulates progenitor cell adherence. We speculate that PACAP may in this manner help restrict adult neural progenitors to the stem cell niche in vivo, with potential significance for aNPC function in physiological and pathological states.
ISSN:1759-0914
1759-0914
DOI:10.1177/1759091417708720