Curcumin-enriched Gemini surfactant nanoparticles exhibited tumoricidal effects on human 3D spheroid HT-29 cells in vitro

Background Here, we examined the tumoricidal effect of Gemini surfactant nanoparticles enriched with curcumin on 3D spheroid HT-29 cells. The delivery of curcumin and other phytocompounds to the tumor niche is an important challenge. Methods Spheroid HT-29 cells were generated by using a conventiona...

Full description

Saved in:
Bibliographic Details
Published in:Cancer nanotechnology 2021-12, Vol.12 (1), p.1-15, Article 3
Main Authors: Zibaei, Zohre, Babaei, Esmaeil, Rezaie Nezhad Zamani, Arezoo, Rahbarghazi, Reza, Azeez, Hewa Jalal
Format: Article
Language:English
Subjects:
3D spheroid culture
Biochemistry
Biomedical Engineering and Bioengineering
Cancer Research
Chemistry and Materials Science
Colorectal cancer
Curcumin
Diameters
Efflux
Flow cytometry
Gemini surfactants
HT-29
Materials Science
Metastasis
Nanoparticles
Nanotechnology
Rhodamine
Spheroids
Surfactants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Here, we examined the tumoricidal effect of Gemini surfactant nanoparticles enriched with curcumin on 3D spheroid HT-29 cells. The delivery of curcumin and other phytocompounds to the tumor niche is an important challenge. Methods Spheroid HT-29 cells were generated by using a conventional hanging drop method and exposed to different concentrations of Gemini-curcumin nanoparticles. The changes in spheroid integrity and cell viability were evaluated by measuring the spheroid diameter and LDH release, respectively. The uptake of Gemini-curcumin nanoparticles was detected by flow cytometry assay. Flow cytometric of Rhodamine 123 efflux was also performed. Migration capacity was analyzed using a Transwell insert assay. By using real-time PCR analysis and Western blotting, we studied the expression level of MMP-2, -9, Vimentin, and E-cadherin genes. Results Gemini-curcumin nanoparticles had the potential to disintegrate spheroids and decrease central density compared to the control group ( p  
ISSN:1868-6958
1868-6966
DOI:10.1186/s12645-020-00074-4