Evaluation of the inflammatory response to Kudoa septempunctata genotype ST3 isolated from olive flounder (Paralichthys olivaceus) in Caco-2 cells

Kudoa septempunctata (Myxosporea, Multivalvulida) is a parasite of the trunk muscle of cultured olive flounder (Paralichthys olivaceus). We investigated whether K. septempunctata genotype ST3 spores induce cell damage and the secretion of inflammatory mediators in Caco-2 cells, which exhibit charact...

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Bibliographic Details
Published in:Parasite (Paris) 2018, Vol.25, p.12-12
Main Authors: Ahn, Meejung, Ko, Hyun Ju, Kim, Jeongtae, Jang, Yeounghwan, Shin, Taekyun
Format: Article
Language:English
Subjects:
Caco-2 cells
Cyclooxygenase-2
Cytotoxicity
Denaturation
Epithelial cells
Fish
Genotype & phenotype
genotype ST3
Genotypes
Inflammation
Inflammatory response
Intestine
Kudoa septempunctata
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactic acid
Lipopolysaccharides
Morphology
Muscles
Myxosporea
Nitric oxide
Nitric-oxide synthase
Paralichthys olivaceus
Prostaglandin E2
Secretion
Spores
Toxicity
Trunk muscles
Valves
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Summary:Kudoa septempunctata (Myxosporea, Multivalvulida) is a parasite of the trunk muscle of cultured olive flounder (Paralichthys olivaceus). We investigated whether K. septempunctata genotype ST3 spores induce cell damage and the secretion of inflammatory mediators in Caco-2 cells, which exhibit characteristics similar to human intestinal epithelial cells. Purified K. septempunctata spores were heated at 95 °C for 5 min. Lactate dehydrogenase (LDH) release was measured to determine the efficacy of denaturation. Naïve and heated spores, lipopolysaccharide (positive control) and vehicle (negative control) were added to Caco-2 cells. Cells were subjected to the cytotoxic LDH assay and western blot analysis to examine the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Supernatants were collected to measure nitric oxide (NO) and prostaglandin E2 (PGE ). Most spores were denaturated by heating, and the spore morphology was found to be wrinkled with shell valves and polar capsules. In addition, cytotoxicity and inflammatory mediators, such as NO, PGE , iNOS, and COX-2, remained unchanged in Caco-2 cells following exposure to naïve and heated spores compared with the positive controls. Collectively, the findings of this study imply that spores of K. septempunctata genotype ST3 do not cause inflammation in Caco-2 cells.
ISSN:1776-1042
1252-607X
1776-1042
DOI:10.1051/parasite/2018013