Transcriptional profiling of skeletal muscle reveals starvation response and compensatory growth in Spinibarbus hollandi

• Published in: BMC genomics 2019-12, Vol.20 (1), p.938-938, Article 938
• Main Authors: Yang, Yang, Zhou, Huiqiang, Hou, Liping, Xing, Ke, Shu, Hu
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Carbohydrate metabolism; Carbohydrates; Cell cycle; Cellular structure; Classification; Compensation; Compensatory growth; Cyprinidae - genetics; Cyprinidae - growth & development; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA replication; Economic conditions; Experiments; Fasting; Fat metabolism; Feeding Behavior; Fish; Fish Proteins - genetics; Food; Gene expression; Gene Expression Profiling - veterinary; Gene Expression Regulation, Developmental; Gene Regulatory Networks; Genes; Genetic aspects; Genomics; Growth; Growth rate; High-Throughput Nucleotide Sequencing - veterinary; Hypertrophy; Information processing; Internal energy; Metabolism; Muscle; Muscle, Skeletal - chemistry; Muscle, Skeletal - growth & development; Muscles; Musculoskeletal system; Next-generation sequencing; Physiological aspects; Physiology; Protein turnover; Proteins; Sequence Analysis, RNA - veterinary; Signal transduction; Skeletal muscle; Spinibarbus hollandi; Starvation response; Transcription; Transcription (Genetics)
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-019-6345-2

Spinibarbus hollandi is an economically important fish species in southern China. This fish is known to have nutritional and medicinal properties; however, its farming is limited by its slow growth rate. In the present study, we observed that a compensatory growth phenomenon could be induced by adequate refeeding following 7 days of fasting in S. hollandi. To understand the starvation response and compensatory growth mechanisms in this fish, the muscle transcriptomes of S. hollandi under control, fasting, and refeeding conditions were profiled using next-generation sequencing (NGS) techniques. More than 4.45 × 10 quality-filtered 150-base-pair Illumina reads were obtained from all nine muscle samples. De novo assemblies yielded a total of 156,735 unigenes, among which 142,918 (91.18%) could be annotated in at least one available database. After 7 days of fasting, 2422 differentially expressed genes were detected, including 1510 up-regulated genes and 912 down-regulated genes. Genes involved in fat, protein, and carbohydrate metabolism were significantly up-regulated, and genes associated with the cell cycle, DNA replication, and immune and cellular structures were inhibited during fasting. After refeeding, 84 up-regulated genes and 16 down-regulated genes were identified. Many genes encoding the components of myofibers were significantly up-regulated. Histological analysis of muscle verified the important role of muscle hypertrophy in compensatory growth. In the present work, we reported the transcriptome profiles of S. hollandi muscle under different conditions. During fasting, the genes involved in the mobilization of stored energy were up-regulated, while the genes associated with growth were down-regulated. After refeeding, muscle hypertrophy contributed to the recovery of growth. The results of this study may help to elucidate the mechanisms underlying the starvation response and compensatory growth.