Autonecrotic Tomato (Solanum lycopersicum L.) Line as a Potential Model for Applications in Proximal Sensing of Biotic and Abiotic Stress

• Published in: Agriculture (Basel) 2024-01, Vol.14 (1), p.136
• Main Authors: Santangelo, Enrico, Giudice, Angelo Del, Figorilli, Simone, Violino, Simona, Costa, Corrado, Bascietto, Marco, Bergonzoli, Simone, Beni, Claudio
• Format: Article
• Language: English
• Subjects: Abiotic stress; Cell death; Chlorophyll; Chromosome 2; Classification; Crop diseases; Genotypes; hybrid necrosis; hyperspectral imaging; Irradiation; Leaf mold; Leaves; Light irradiation; Morphology; Mutants; Necrosis; Pathogens; phenotyping; Plant diseases; Plant growth; Plants; precision farming; Prediction models; Proteins; Remote sensing; Spectral reflectance; Statistical analysis; Stresses; Tomatoes
• ISSN: 2077-0472; 2077-0472
• DOI: 10.3390/agriculture14010136

The autonecrotic tomato line V20368 (working code IGSV) spontaneously develops necrotic lesions with acropetal progression in response to an increase in temperature and light irradiation. The process is associated with the interaction between tomato and Cladosporium fulvum, the fungal agent of leaf mold. The contemporary presence of an in-house allele encoding the Rcr3lyc protein and the resistance gene Cf-2pim (from Solanum pimpinellifolium) causes auto-necrosis on the leaves even in the absence of the pathogen (hybrid necrosis). The aim of the work was (i) to examine the potential value of the necrotic genotype as a model system for setting up theoretical guidance for monitoring the phytosanitary status of tomato plants and (ii) to develop a predictive model for the early detection of pathogens (or other stresses) in the tomato or other species. Eighteen IGSV tomato individuals at the 4–5th true-leaf stage were grown in three rows (six plants per row) considered to be replicates. The healthy control was the F1 hybrid Elisir (Olter). A second mutant line (SA410) deriving from a cross between the necrotic mutant and a mutant line of the lutescent (l) gene was used during foliar analysis via microspectrometry. The leaves of the mutants and normal plants were monitored through a portable VIS/NIR spectrometer SCIOTM (Consumer Physics, Tel Aviv, Israel) covering a spectral range between 740 and 1070 nm. Two months after the transplant, the acropetal progression of the autonecrosis showed three symptomatic areas (basal, median, apical) on each IGSV plant: necrotic, partially damaged, and green, respectively. Significantly lower chlorophyll content was found in the basal and median areas of IGSV compared with the control (Elisir). A supervised classification/modelling method (SIMCA) was used. Applying the SIMCA model to the dataset of 162 tomato samples led to the identification of the boundary between the healthy and damaged samples (translational critical distance). Two 10 nm wavelength ranges centred at 865 nm and 1055 nm exhibited a stronger link between symptomatology and spectral reflectance. Studies on specific highly informative mutants of the type described may allow for the development of predictive models for the early detection of pathogens (or other stresses) via proximal sensing.