Incidence Assessment of MTHFR C677T and A1298C Polymorphisms in Iranian Non-syndromic Cleft Lip and/or Palate Patients

Background and aims. The aim of the present study is to determine the incidence of MTHFR C677 T and A1298C muta-tions in Iranian patients with cleft lip and/or cleft palate. Materials and methods. We screened 61 Iranian patients with cleft lip and/or cleft palate for mutations in the two alleles of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dental research, dental clinics, dental prospects dental clinics, dental prospects, 2015-01, Vol.9 (2), p.101-104
Main Authors: Ebadifar, Asghar, Ameli, Nazila, Khorramkhorshid, Hamid Reza, Salehi Zeinabadi4, Mehdi, Kamali, Kourosh, Khoshbakht, Tayyebeh
Format: Article
Language:English
Subjects:
A1298C
C677T
Cardiovascular disease
Defects
Enzymes
Genes
Homocysteine
Metabolism
methylenetetrahydrofolate reductase
Mutation
Original Research
orofacial cleft
polymorphism
Population
Questionnaires
Studies
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background and aims. The aim of the present study is to determine the incidence of MTHFR C677 T and A1298C muta-tions in Iranian patients with cleft lip and/or cleft palate. Materials and methods. We screened 61 Iranian patients with cleft lip and/or cleft palate for mutations in the two alleles of MTHFR gene associated with cleft lip and/or palate: A1298C and C677T, using Polymerase Chain Reaction following by RFLP. Results. The 677T and 1298C homozygote genotypes showed a frequency of 36.1% and 11.4%, respectively. Combined genotype frequencies in newborns having oral clefts showed that the highest genotype was 677TT/1298AA (22.9%) and 677TT/1298CC genotypes were not observed. Conclusion. The results showed that 65.6% of all patients had at least one T mutant allele in C677T and 58.9% C mutant allele for A1298C. According to the frequencies of homozygosity of mutant alleles, it could be said that MTHFR genotype of 677TT shows a greater role in having oral clefts.
ISSN:2008-210X
2008-2118
DOI:10.15171/joddd.2015.020