A Novel Medium for Isolating Two Japanese Species in the Fusarium graminearum Species Complex and a Dipstick DNA Chromatography Assay for Species Identification and Trichothecene Typing

Members of the Fusarium graminearum species complex (Fg complex) are the primary pathogens that cause Fusarium head blight in wheat and barley. Fg complex members grow poorly on Fusarium oxysporum-selective media, such as Komada and Fo-G2, that have also been used for the isolation of other Fusarium...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2022-10, Vol.8 (10), p.1048
Main Authors: Suga, Haruhisa, Hayashi, Masahiro, Kushiro, Masayo, Miyano, Norichika, Inoue, Hiroyoshi, Nakajima, Kaori, Kawakami, Taku, Tonooka, Takuji, Nakajima, Takashi, Shimizu, Masafumi, Kageyama, Koji
Format: Article
Language:English
Subjects:
Barley
cereal disease
Chromatography
Deoxyribonucleic acid
Dextrose
Disease control
DNA
Fusarium
Fusarium graminearum
Fusarium head blight
Laboratories
molecular diagnosis
multiplex PCR
mycotoxin producer
Pathogens
Pentachloronitrobenzene
plant pathogen
Ribosomal DNA
Selective media
Species
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Summary:Members of the Fusarium graminearum species complex (Fg complex) are the primary pathogens that cause Fusarium head blight in wheat and barley. Fg complex members grow poorly on Fusarium oxysporum-selective media, such as Komada and Fo-G2, that have also been used for the isolation of other Fusarium species. Therefore, Komada medium was modified as FG medium for the isolation of Fg complex members. However, the production of pentachloronitrobenzene that is the most effective component of FG medium is discontinued and new media is required for the selective isolation of Fg complex members. In addition, the rapid diagnosis of isolated fungi is useful for the disease control. Novel tools have been developed for isolating and characterizing Fg complex members. FG21, a semi-selective medium for isolating Fg complex members, was developed using potato dextrose agar. Furthermore, a dipstick DNA chromatography assay was developed both to identify Fusarium graminearum sensu stricto and Fusarium asiaticum in the Fg complex and their trichothecene mycotoxin types. The easier isolation and characterization of Fg complex members in Japan was attained by the combined use of FG21 medium and the dipstick DNA chromatography assay.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8101048