Anti-Osteoporosis Effects of the Eleutherococcus senticosus , Achyranthes japonica , and Atractylodes japonica Mixed Extract Fermented with Nuruk

• Published in: Nutrients 2021-10, Vol.13 (11), p.3904
• Main Authors: Eun, So Young, Cheon, Yoon-Hee, Park, Gyeong Do, Chung, Chong Hyuk, Lee, Chang Hoon, Kim, Ju-Young, Lee, Myeung Su
• Format: Article
• Language: English
• Subjects: Achyranthes; Achyranthes japonica; Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid resistance; Actin; AKT protein; Animals; Atractylodes; Atractylodes japonica; Biocompatibility; Biomedical materials; Bone diseases; Bone growth; Bone loss; Bone marrow; bone resorption; Bone Resorption - prevention & control; c-Fos protein; Cell Differentiation - drug effects; Cytotoxicity; Eleutherococcus; Eleutherococcus senticosus; Fermentation; Fractures; Gene expression; Herbal medicine; Inflammatory response; Lipopolysaccharides; Male; Medical research; Mice; Mice, Inbred ICR; mRNA; nuruk fermentation; Osteoclastogenesis; Osteoclasts; Osteoclasts - drug effects; Osteoclasts - physiology; Osteogenesis - drug effects; Osteoporosis; Osteoporosis - prevention & control; Phosphorylation; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; RANK Ligand - metabolism; Signal Transduction; TRANCE protein; Vigeo
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13113904

Vigeo is a mixture of fermented extracts of Maxim (ESM), (Miq.) Nakai (AJN), and Koidzumi (AJK) manufactured using the traditional Korean nuruk fermentation method. Although the bioactive effects of ESM, AJN, and AJK have already been reported, the pharmacological effects of Vigeo have not been proven. Therefore, in this study, we investigated whether Vigeo had inhivitory effects on lipopolysaccharide (LPS)-induced inflammatory bone loss in vivo and receptor activator of nuclear factor-B ligand (RANKL)-induced osteoclastogenesis and the related mechanism in vitro. Vigeo administration conferred effective protection against bone loss induced by excessive inflammatory response and activity of osteoclasts in LPS-induced inflammatory osteoporosis mouse model. In addition, Vigeo significantly suppressed the formation of tartrate-resistant acid phosphatase-positive osteoclasts induced by RANKL and inhibited F-actin formation and bone resorbing activity without any cytotoxicity. Moreover, Vigeo significantly inhibited RANKL-induced phosphorylation of p38, ERK, JNK, IκB, and AKT and degradation of IkB. Additionally, Vigeo strongly inhibited the mRNA and protein expression of c-FOS and NFATc1 and subsequently attenuated the expression of osteoclast specific marker genes induced by RANKL. We demonstrated for the first time the anti-osteoporosis effect of Vigeo, suggesting that it could be a potential therapeutic candidate for the treatment of osteoclast-mediated inflammatory bone diseases.