Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins. Protein A purification is specific and efficient. However, there still remain several issues to be addressed, such as incomplete...

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Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.1827-1827, Article 1827
Main Authors: Imura, Yuichi, Tagawa, Toshiaki, Miyamoto, Yuya, Nonoyama, Satoshi, Sumichika, Hiroshi, Fujino, Yasuhiro, Yamanouchi, Masaya, Miki, Hideo
Format: Article
Language:English
Subjects:
631/45/612
631/61/51/1568
639/638/11
Affinity
Affinity chromatography
Chromatography
Fc receptors
Fusion protein
Humanities and Social Sciences
Impurities
Monoclonal antibodies
multidisciplinary
Physicochemical properties
Protein A
Protein purification
Proteins
Science
Science (multidisciplinary)
Shelf life
Thiols
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Summary:Protein A affinity chromatography has been widely used for both laboratory scale purification and commercial manufacturing of monoclonal antibodies and Fc-fusion proteins. Protein A purification is specific and efficient. However, there still remain several issues to be addressed, such as incomplete clearance of impurities including host cell proteins, DNA, aggregates, etc. In addition, the effects of wash buffers in protein A purification on the physicochemical characteristics of antibodies have yet to be fully understood. Here we found a new purification protocol for monoclonal antibodies that can improve physicochemical properties of monoclonal antibodies simply by inserting an additional wash step with a basic buffer after the capture step to the conventional protein A purification. The effects of the alkaline wash on monoclonal antibodies were investigated in terms of physicochemical characteristics, yields, and impurity clearance. The simple insertion of an alkaline wash step resulted in protection of antibodies from irreversible aggregation, reduction in free thiols and impurities, an improvement in colloidal and storage stability, and enhanced yields. This new procedure is widely applicable to protein A affinity chromatography of monoclonal antibodies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-81366-6