Genome-Wide Analysis and Expression Profiling of Soybean RbcS Family in Response to Plant Hormones and Functional Identification of GmRbcS8 in Soybean Mosaic Virus

• Published in: International journal of molecular sciences 2024-09, Vol.25 (17), p.9231
• Main Authors: Zhou, Fangxue, Feng, Wenmi, Mou, Kexin, Yu, Zhe, Zeng, Yicheng, Zhang, Wenping, Zhou, Yonggang, Li, Yaxin, Gao, Hongtao, Xu, Keheng, Feng, Chen, Jing, Yan, Li, Haiyan
• Format: Article
• Language: English
• Subjects: Amino acids; Chloroplasts; Chromosomes; Disease Resistance - genetics; Enzymes; expression analysis; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genome-Wide Association Study; genome-wide characterization; Genomes; Genomics; Glycine max - genetics; Glycine max - metabolism; Glycine max - virology; Localization; Multigene Family; Phylogenetics; Phylogeny; Plant Diseases - genetics; Plant Diseases - virology; Plant growth; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plant Proteins - genetics; Plant Proteins - metabolism; Potyvirus - pathogenicity; Proteins; RbcS gene family; Ribulose-Bisphosphate Carboxylase - genetics; Ribulose-Bisphosphate Carboxylase - metabolism; Rice; soybean; soybean mosaic virus; Soybeans
• ISSN: 1661-6596; 1422-0067; 1422-0067
• DOI: 10.3390/ijms25179231

Rubisco small subunit (RbcS), a core component with crucial effects on the structure and kinetic properties of the Rubisco enzyme, plays an important role in response to plant growth, development, and various stresses. Although genes have been characterized in many plants, their muti-functions in soybeans remain elusive. In this study, a total of 11 genes were identified and subsequently divided into three subgroups based on a phylogenetic relationship. The evolutionary analysis revealed that whole-genome duplication has a profound effect on . The cis-acting elements responsive to plant hormones, development, and stress-related were widely found in the promoter region. Expression patterns based on the RT-qPCR assay exhibited that genes are expressed in multiple tissues, and notably ( ) exhibited the highest expression level compared to other members, especially in leaves. Moreover, differential expressions of genes were found to be significantly regulated by exogenous plant hormones, demonstrating their potential functions in diverse biology processes. Finally, the function of in enhancing soybean resistance to soybean mosaic virus (SMV) was further determined through the virus-induced gene silencing (VIGS) assay. All these findings establish a strong basis for further elucidating the biological functions of genes in soybeans.