Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation

This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV)....

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Published in:Orbital : The Electronic Journal of Chemistry 2019-09, Vol.11 (4), p.277-283
Main Authors: Thais Silva Alves, Antonio Rogério Fiorucci, Gilberto José Arruda
Format: Article
Language:English
Subjects:
carbon paste electrode
determination
hormones
medications
voltammetry
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Antonio Rogério Fiorucci
Gilberto José Arruda
description This paper describes a simple, inexpensive, highly sensitive, and efficient electrochemical method to determine Estradiol in pharmaceutical formulations. The oxidation reaction on the electrode surface was electrochemically characterized by cyclic voltammetry (CV) and square wave voltammetry (SWV). The investigation of Estradiol at carbon paste electrode revealed a non-reversible oxidation peak at +640 mV vs. Ag/AgCl, which was used for electrochemical detection of Estradiol. The operating parameters (pH, frequency, step potential, and amplitude) were optimized in relation to the peak current intensity, and a calibration curve was set up in a concentration range of 0.059 - 2.997 mg L-1, with a detection limit of 21.85 µg L-1. After calibration curve was plotted, the developed procedure was applied to determine Estradiol in parmacheutical formulation. These results show that the proposed method can be used for Estradiol quantification in pharmaceutical formulations with high sensitivity, specificity, stability, and reproducibility, and can be applied in analytical routines in laboratories of quality control analysis in drugs. DOI: http://dx.doi.org/10.17807/orbital.v11i4.1332
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subjects carbon paste electrode
determination
hormones
medications
voltammetry
title Development of an Electrochemical Methodology for the Quantification of β-estradiol in Pharmaceutical Formulation
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