Reduced gonadotroph stimulation by ethanolamine plasmalogens in old bovine brains

Ethanolamine plasmalogens (EPls), unique alkenylacyl-glycerophospholipids, are the only known ligands of G-protein-coupled receptor 61—a novel receptor co-localised with gonadotropin-releasing hormone receptors on anterior pituitary gonadotrophs. Brain EPl decreases with age. Commercial EPl—extracte...

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Bibliographic Details
Published in:Scientific reports 2021-02, Vol.11 (1), p.4757-4757, Article 4757
Main Authors: Kadokawa, Hiroya, Kotaniguchi, Miyako, Kereilwe, Onalenna, Kitamura, Shinichi
Format: Article
Language:English
Subjects:
631/443/494/2732
631/601/1737
Acyltransferase
Age
Biosynthesis
Brain
Cattle
Ethanolamine
Follicle-stimulating hormone
G protein-coupled receptors
Gonadotropin-releasing hormone
Gonadotropin-releasing hormone receptors
Gonadotropins
Humanities and Social Sciences
Hypothalamus
Infertility
Liquid chromatography
Mass spectrometry
Mass spectroscopy
multidisciplinary
Pituitary (anterior)
Polymerase chain reaction
Reverse transcription
Science
Science (multidisciplinary)
Secretion
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Summary:Ethanolamine plasmalogens (EPls), unique alkenylacyl-glycerophospholipids, are the only known ligands of G-protein-coupled receptor 61—a novel receptor co-localised with gonadotropin-releasing hormone receptors on anterior pituitary gonadotrophs. Brain EPl decreases with age. Commercial EPl—extracted from the cattle brain (unidentified age)—can independently stimulate FSH secretion from gonadotrophs. We hypothesised that there exists an age-related difference in the quality, quantity, and ability of bovine brain EPls to stimulate bovine gonadotrophs. We compared the brains of young (about 26 month old heifers) and old (about 90 month old cows) Japanese Black bovines, including EPls obtained from both groups. Additionally, mRNA expressions of the EPl biosynthesis enzymes, glyceronephosphate O-acyltransferase, alkylglycerone phosphate synthase, and fatty acyl-CoA reductase 1 (FAR1) were evaluated in young and old hypothalami. The old-brain EPl did not stimulate FSH secretion from gonadotrophs, unlike the young-brain EPl. Molecular species of EPl were compared using two-dimensional liquid chromatography-mass spectrometry. We identified 20 EPl molecular species of which three and three exhibited lower ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-84306-6